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Enhanced In situ Activity of Peroxidases and Lignification of Root Tissues after Exposure to Non-Thermal Plasma Increases the Resistance of Pea Seedlings

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Abstract

Improving the germination of economically important crops and the condition of young plants is a major challenge currently facing agricultural practice. Pea (Pisum sativum L.) is one of the four most important cultivated legumes, along with groundnut (Arachis hypogaea L.), soybean (Glycine max L.) and beans (Phaseolus vulgaris L.). Due to the high protein content (23–33%), there is an interest in growing this crop as a source of protein for humans and animals. In this study, we focused on the effect of Cold Atmospheric Pressure Plasma (CAPP) on the decontamination and germination of pea seeds, on young seedling growth and production parameters, and on increasing their resistance and mechanical strength. We can state that germination increased by 10 to 25% after plasma treatment, and the most significant decontamination effect was detected when using non-thermal plasma generated in the ambient air (A-variants) and in the nitrogen atmosphere (N-variants). The increased in situ activity of peroxidases (POX) in the cell walls of A-variants and N-variants is also closely related to the increase in the mechanical strength of the cell walls and thus contributes to the higher resistance of these seedlings. This is also illustrated by the differences in lignin deposition among the different variants after CAPP treatment. To our knowledge, this is the first study concerning the influence of CAPP on the lignification of root tissues and on increasing the strength and resistance of plants.

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Acknowledgement

This work was supported by the Slovak Research and Development Agency under the contract No. APVV-16-0216 and VEGA No. 1/0410/18. Authors would like to express thanks doc. RNDr. Anna Zahoranová, PhD. for help and many valuable comments.

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Authors and Affiliations

  1. Department of Plant Physiology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 842 15, Bratislava 4, Mlynská dolina, Slovakia

    Švubová Renáta, Válková Nicolette, Bathoova Monika & Slováková Ľudmila

  2. Department of Genetics, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 842 15, Bratislava 4, Mlynská dolina, Slovakia

    Kyzek Stanislav & Gálová Eliška

  3. Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, 842 48, Bratislava 4, Mlynská dolina, Slovakia

    Medvecká Veronika

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  1. Švubová Renáta
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Renáta, Š., Nicolette, V., Monika, B. et al. Enhanced In situ Activity of Peroxidases and Lignification of Root Tissues after Exposure to Non-Thermal Plasma Increases the Resistance of Pea Seedlings. Plasma Chem Plasma Process 41, 903–922 (2021). https://doi.org/10.1007/s11090-021-10160-z

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