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Regulation of S100B Expression in Long-Term Potentiation

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The role of intracellular regulatory cascades in inducing the expression of S100B during the formation of long-term post-tetanic potentiation (LTP) in field CA1 was studied in hippocampal slices from rats. Activation of transcription factor p53 (a positive regulator of S100B transcription) using nutlin-3 increased the basal level of S100B mRNA to 151% of control, which was significantly lower than its content in tetanized slices (280%). Thus, p53 cannot be the only transcription factor regulating S100B expression in LTP. KN-93, an inhibitor of Ca2+/calmodulin-dependent kinases (CaMK), completely blocked the increase in the S100B mRNA level after tetanization, while its inactive analog KN-92 had no effect on S100B expression. K-252a, an inhibitor of CaMKII and receptor tyrosine kinases, significantly suppressed S100B expression in LTP, while inhibition of MAPK p38 or RSK2 produced moderate decreases and inhibition of MEK1 had no effect on the quantity of S100B mRNA. Thus, calmodulin kinases play a key role in inducing S100B expression in LTP.

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Authors and Affiliations

  1. Design Technological Institute of Digital Techniques, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    P. D. Lisachev

  2. Institute of Molecular Biology and Biophysics, Siberian Branch, Russian Academy of Medical Sciences, Novosibirsk, Russia

    V. O. Pustyl’nyak & M. B. Shtark

  3. Novosibirsk State University, Novosibirsk, Russia

    V. O. Pustyl’nyak

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  1. P. D. Lisachev
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Correspondence to P. D. Lisachev.

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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 100, No. 8, pp. 953–963, August, 2014.

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Lisachev, P.D., Pustyl’nyak, V.O. & Shtark, M.B. Regulation of S100B Expression in Long-Term Potentiation. Neurosci Behav Physi 46, 312–318 (2016). https://doi.org/10.1007/s11055-016-0235-8

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