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Cerium oxide nanoparticles protect primary mouse bone marrow stromal cells from apoptosis induced by oxidative stress

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Abstract

Cerium oxide nanoparticles (nanoceria) have been widely used in industries and biomedical fields due to its unique properties. Previous biodistribution studies of nanoceria in vivo have shown that they are accumulated in the bone of mice after intravenous administration, about 20 % of the total intake, however, the potential effect and the mechanism of nanoceria on bone metabolism are not well-understood. Our results showed that both 25 and 50 nm nanceria decreased the damage of cell viability induced by H2O2 in a dose-dependent manner. The apoptosis ratio of pre-incubated group with nanoceria was lower than the H2O2 group. The cellular uptake studies indicated that there was a dose-dependent accumulation of both two size nanoparticles in bone marrow stromal cells. Nanoceria could be uptaken by cells due to the synergistic effect of multiple endocytosis mechanisms, and then evenly distributed in the cytoplasm without entering the nucleus. Our results suggest that nanoceria could reduce intracellular ROS level induced by H2O2 in a dose-dependent manner, moreover, maintain the normal function of mitochondria, suggesting nanoceria may have potent applications for preventing or treating osteoporosis.

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Acknowledgments

This work was supported by Chinese Natural Science Foundation project (No. 21271059) and Research Fund for the Doctoral Program of Higher Education of China (No. 20111301110004), Hebei Province “Hundred Talents Program” (BR2–202), Training Program for Innovative Research Team, and Leading Talent in Hebei Province University (LJRC024).

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Authors and Affiliations

  1. College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Hebei University, Baoding, People’s Republic of China

    Qun Zhang, Kun Ge, Jianlei Duan, Shizhu Chen, Ran Zhang, Cuimiao Zhang, Shuxiang Wang & Jinchao Zhang

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  1. Qun Zhang
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  2. Kun Ge
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  3. Jianlei Duan
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  4. Shizhu Chen
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  5. Ran Zhang
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  7. Shuxiang Wang
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  8. Jinchao Zhang
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Correspondence to Jinchao Zhang.

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Qun Zhang, Kun Ge contributed equally to this work.

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Zhang, Q., Ge, K., Duan, J. et al. Cerium oxide nanoparticles protect primary mouse bone marrow stromal cells from apoptosis induced by oxidative stress. J Nanopart Res 16, 2697 (2014). https://doi.org/10.1007/s11051-014-2697-3

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