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Role of antioxidant systems, lipid peroxidation, and nitric oxide in postmenopausal osteoporosis

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Abstract

In this study we assessed activities of antioxidant enzymes, lipid peroxidation end-products, and nitric oxide (NO) levels in women with postmenopausal osteoporosis (PMO). Relationship between oxidative stress parameters and NO levels with bone mineral density (BMD) and clinical variables influencing bone mass and health related quality of life measures was also investigated in women with PMO.

Postmenopausal women (n = 87), aged 40–65, without previous diagnosis or treatment for osteoporosis and independent in daily living activities were included. BMD was measured at the lumbar spine and proximal femur using dual-X-ray absorptiometry (DXA). Erythrocyte catalase (CATe) enzyme activity, erythrocyte and plasma enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and lipid peroxidation end-product malondialdehyde (MDA) and nitrite/nitrate levels, by product of NO were studied. A total of 23 healthy non-porotic women were included as controls.

Women with PMO had significantly lower erythrocyte CATe enzyme activity and higher erythrocyte malondialdehyde (MDAe) and erythrocyte nitric oxide (NOe) levels in comparison to controls whereas erythrocyte SODe and GSH-Px enzyme activity was similar. In plasma, osteporotic women had significantly higher SOD enzyme activity and higher MDA levels whereas similar GSH-Px enzyme activity and NO levels compared to non-porotic controls. Significant correlation was found between erythrocyte SODe, CATe enzyme activity and NOe levels with proximal femur BMD. Some of the quality of life scores as pain, mental, and social functions correlated with antioxidant enzyme activities and NO levels.

Consequently, oxidative stress markers may be an important indicator for bone loss in postmenopausal women. Further researches assessing the oxidative stress markers and NO in bone tissue and changes with anti-osteoporotic drugs would be valuable to better understand the role of free radicals, antioxidants, and NO in the regulation of bone mass.

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Abbreviations

ROS:

Reactive oxygen species

NOS:

Nitric oxide synthase

nNOS:

Neuronal nitric oxide synthase

iNOS:

Inducible nitric oxide synthase

eNOS:

Endothelial nitric oxide synthase

PMO:

Postmenopausal osteoporosis

DXA:

Dual-X-ray absorptiometry

QUALEFFO:

Quality of Life Questionnaire of the European Foundation for Osteoporosis-41 item

CTx:

Type I cross-linked C-telopeptide

BSO:

L-buthionine-(S,R)-sulfoximine

ONOO− :

Peroxynitrite

CATe:

Erythrocyte catalase

SODe:

Erythrocyte superoxide dismutase

MDAe:

Erythrocyte malondialdehyde

GSH-Pxe:

Erythrocyte glutathione peroxidase

NOe:

Erythrocyte nitric oxide

BMD:

Bone mineral density

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Authors and Affiliations

  1. Division of Rheumatology, Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Firat University, Firat Tip Merkezi, FTR AD, Romatoloji BD, 23119, Elazig, Turkey

    Salih Ozgocmen, Huseyin Kaya & Rabia Aydogan

  2. Department of Physiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey

    Ersin Fadillioglu

  3. Department of Physiology, Faculty of Medicine, Inonu University, Malatya, Turkey

    Zumrut Yilmaz

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  1. Salih Ozgocmen
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  2. Huseyin Kaya
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  3. Ersin Fadillioglu
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  4. Rabia Aydogan
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  5. Zumrut Yilmaz
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Correspondence to Salih Ozgocmen.

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Ozgocmen, S., Kaya, H., Fadillioglu, E. et al. Role of antioxidant systems, lipid peroxidation, and nitric oxide in postmenopausal osteoporosis. Mol Cell Biochem 295, 45–52 (2007). https://doi.org/10.1007/s11010-006-9270-z

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  • DOI: https://doi.org/10.1007/s11010-006-9270-z

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