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Phosphatidylcholine nanovesicles coated with chitosan or chondroitin sulfate as novel devices for bacteriocin delivery

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Abstract

There is increased interest on the use of natural antimicrobial peptides in biomedicine and food preservation technologies. In this study, the antimicrobial activity of nisin encapsulated into nanovesicles containing polyanionic polysaccharides was investigated. Nisin was encapsulated in phosphatidylcholine (PC) liposomes containing chitosan or chondroitin sulfate by the thin-film hydration method and tested for antimicrobial activity against Listeria spp. The mean particle size of PC liposomes was 145 nm and varied to 210 and 134 nm with the incorporation of chitosan and chondroitin sulfate, respectively. Nisin-containing nanovesicles with and without incorporation of polysaccharides had a zeta potential values around −20 mV, showing mostly spherical structures when observed by transmission electron microscopy. Encapsulated nisin had similar efficiency as free nisin in inhibiting Listeria spp. isolated from bovine carcass, and greater efficiency in inhibiting Listeria monocytogenes. The formulation containing chitosan was more stable and more efficient in inhibiting L. monocytogenes when compared to the other nanovesicles tested. After 24 h, the viable cell counts were 2 log lower as compared with the other treatments and 7 log comparing to controls.

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Acknowledgments

This work received financial support of the Brazilian Agencies CNPq and CAPES.

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Authors and Affiliations

  1. Laboratorio de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul (ICTA-UFRGS), Av. Bento Gonçalves 9500, Porto Alegre, 91501-970, Brazil

    Indjara Mallmann da Silva, Juliana Ferreira Boelter & Adriano Brandelli

  2. Laboratorio de Instrumentação e Dinâmica Molecular, Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, 91501-970, Brazil

    Nádya Pesce da Silveira

Authors
  1. Indjara Mallmann da Silva
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  2. Juliana Ferreira Boelter
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  3. Nádya Pesce da Silveira
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  4. Adriano Brandelli
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Correspondence to Adriano Brandelli.

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da Silva, I.M., Boelter, J.F., da Silveira, N.P. et al. Phosphatidylcholine nanovesicles coated with chitosan or chondroitin sulfate as novel devices for bacteriocin delivery. J Nanopart Res 16, 2479 (2014). https://doi.org/10.1007/s11051-014-2479-y

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  • DOI: https://doi.org/10.1007/s11051-014-2479-y

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