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Transcriptional patterns of human retinal pigment epithelial cells under protracted high glucose

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Abstract

Background

The retinal pigment epithelium (RPE) is essential for retinal homeostasis. Comprehensively exploring the transcriptional patterns of diabetic human RPE promotes the understanding of diabetic retinopathy (DR).

Methods and Results

A total of 4125 differentially expressed genes (DEGs) were screened out from the human primary RPE cells subjected to prolonged high glucose (HG). The subsequent bioinformatics analysis is divided into 3 steps. In Step 1, 21 genes were revealed by intersecting the enriched genes from the KEGG, WIKI, and Reactome databases. In Step 2, WGCNA was applied and intersected with the DEGs. Further intersection based on the enrichments with the GO biological processes, GO cellular components, and GO molecular functions databases screened out 12 candidate genes. In Step 3, 13 genes were found to be simultaneously up-regulated in the DEGs and a GEO dataset involving human diabetic retinal tissues. VEGFA and ERN1 were the 2 starred genes finally screened out by overlapping the 3 Steps.

Conclusion

In this study, multiple genes were identified as crucial in the pathological process of RPE under protracted HG, providing potential candidates for future researches on DR. The current study highlights the importance of RPE in DR pathogenesis.

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Data availability

All data supporting the findings of this study are available within the paper and its Supplementary Information.

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Acknowledgements

Not applicable.

Funding

The work was supported by the National Natural Science Foundation of China (No. 81670885), the Natural Science Foundation of Guangdong Province of China (No. 2021A1515010513), and the Initial Scientific Research Fund of Doctors in Zhuzhou Hospital Affiliated to Xiangya School of Medicine Central South University (No. n/a).

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Authors and Affiliations

  1. Department of Ophthalmology, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, 116 South Changjiang Road, Zhuzhou, 412000, China

    Hao Huang, Jianjun Yan & Wei Liu

  2. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat-Sen University, Guangzhou, 510000, China

    Hao Huang, Jingshu Zeng, Xielan Kuang & Huangxuan Shen

  3. Biobank of Eye, State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, 54 Xianlie Road, Guangzhou, 510000, China

    Xielan Kuang & Huangxuan Shen

  4. Amass Ophthalmology, Guangzhou, 510000, China

    Fan He

  5. Eye Center of Xiangya Hospital, Central South University, Changsha, 410000, China

    Bowen Li

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  1. Hao Huang
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  2. Jingshu Zeng
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  4. Fan He
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  7. Wei Liu
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  8. Huangxuan Shen
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Contributions

HH and HS conceived and designed the study. HH and JZ conducted the experiments. XK, FH, JY, and BL contributed to the data analysis. WL contributed to the image processing. HH and WL drafted the manuscript. All the authors revised the manuscript and approved its submission.

Corresponding authors

Correspondence to Wei Liu or Huangxuan Shen.

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Competing interests

The authors have no relevant financial or non-financial interests to disclose.

Ethics approval

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Institutional Review Board and the Medical Ethics Committee of State Key laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University (Approval NO. 2021KYPJ196).

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Informed consents for use in scientific research were obtained from the donors or donors’ families by the Eye Bank of Guangdong Province.

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Huang, H., Zeng, J., Kuang, X. et al. Transcriptional patterns of human retinal pigment epithelial cells under protracted high glucose. Mol Biol Rep 51, 477 (2024). https://doi.org/10.1007/s11033-024-09479-5

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