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Investigation of the protective effects of piceatannol on experimental subarachnoid hemorrhage in rats

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Abstract

Background

Subarachnoid hemorrhage (SAH) is one of the most prevalent brain injuries in humans which has poor prognosis and high mortality rates. Due to several medical or surgical treatment methods, a gold standard method doesn’t exist for SAH treatment. Piceatannol (PCN), a natural analog of resveratrol, was reported to reduce inflammation and apoptosis promising a wide range of therapeutic alternatives. In this study, we aimed to investigate the effects of PCN in an experimental SAH model. The alleviating effects of PCN in the hippocampus in an experimental SAH model were investigated for the first time.

Methods and results

In this study, 27 Wistar Albino male rats (200–300 g; 7–8 week) were used. Animals were divided into three groups; SHAM, SAH, and SAH + PCN. SAH model was created with 120 µl of autologous arterial tail blood to prechiasmatic cisterna. 30 mg/kg PCN was administered intraperitoneally at 1st h after SAH. Neurological evaluation was performed with Garcia's score. RT-PCR was performed for gene expression levels in the hippocampus. Pyknosis, edema, and apoptosis were evaluated by H&E and TUNEL staining. Our results indicated that PCN administration reduced apoptosis (P < 0.01), cellular edema, and pyknosis (P < 0.05) in the hippocampus after SAH. Moreover, PCN treatment significantly decreased the expression levels of TNF-α (P < 0.01), IL-6 (P < 0.05), NF-κB (P < 0.05), and Bax (P < 0.05) in the hippocampus.

Conclusions

Our results demonstrated that PCN might be a potential therapeutic adjuvant agent for the treatment of early brain injury (EBI) following SAH. Further studies are required to clarify the underlying mechanisms and treatment options of SAH.

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Data availability

The datasets are available from the corresponding author upon reasonable request.

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Acknowledgements

This article was partly produced from the master of science thesis of the first author.

Funding

This work was supported by the Scientific Research Coordination Unit [BAPD] of Çanakkale Onsekiz Mart University, Çanakkale, Turkey (Project Number: TYL-2021-3406).

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Çanakkale Onsekiz Mart University, Sevim Buluç street Terzioğlu Campus, 17100, Çanakkale, Turkey

    Gülfem Erbil & Metehan Uzun

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  1. Gülfem Erbil
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All authors contributed equally to this study.

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Correspondence to Metehan Uzun.

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The Authors declare that there is no conflict of interest.

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This study was approved by the Ethics Committee of Çanakkale Onsekiz Mart University, Turkey. (Decision number protocol number: 2020-E.2000168654).

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Erbil, G., Uzun, M. Investigation of the protective effects of piceatannol on experimental subarachnoid hemorrhage in rats. Mol Biol Rep 51, 366 (2024). https://doi.org/10.1007/s11033-024-09275-1

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