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Screening and identification of photoresponse factors in kiwifruit (Actinidia arguta) development

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Abstract

Background

Light is essential for kiwifruit development, in which photoresponse factors contributes greatly to the quality formation. ‘Light sensitive hypocotyls, also known as light-dependent short hypocotyls’ (LSH) gene family can participate in fruit development as photoresponse factor. However, the key LSH gene that determine kiwifruit development remains unclear. This study aim to screen and identify the key gene AaLSH9 in A. arguta.

Materials and methods

Genome-wide identification of the LSH gene family was used to analyse LSH genes in kiwifruit. Homologous cloning was used to confirm the sequence of candidate LSH genes. qRT-PCR and cluster analysis of expression pattern were used to screen the key AaLSH9 gene. Subcellular localization of AaLSH9 in tobacco leaves and overexpression of AaLSH9 in Arabidopsis thaliana hy5 mutant plants were used to define the acting place in cell and identify molecular function, respectively.

Results

We identified 15 LSH genes, which were divided into two sub-families namely A and B. Domain analysis of A and B showed that they contained different domain organizations, which possibly played key roles in the evolution process. Three LSH genes, AaLSH2, AaLSH9, and AaLSH11, were successfully isolated from Actinidia arguta. The expression pattern and cluster analysis of these three AaLSH genes suggested AaLSH9 might be a key photoresponse gene participating in fruit development in A. arguta. Subcellular localization showed AaLSH9 protein was located in the nucleus. The overexpression of AaLSH9 gene in Arabidopsis thaliana hy5 mutant plants partially complemented the long hypocotyls of hy5 mutant, implying AaLSH9 played a key role as photoresponse factor in cells. In addition, the seed coat color of A. thaliana over-expressing AaLSH9 became lighter than the wide type A.thaliana. Finally, AaCOP1 was confirmed as photoresponse factor to participate in developmental process by stable transgenic A. thaliana.

Conclusions

AaLSH9 can be involved in kiwifruit (A. arguta) development as key photoresponse factor. Our results not only identified the photoresponse factors AaLSH9 and AaCOP1 but also provided insights into their key role in fruit quality improvement in the process of light response.

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Data availability

The datasets utilized and/or analyzed in the present study are available from the corresponding author on reasonable request.

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Acknowledgements

Here we thank Professor Huiyong Zhang of Henan Agricultural University for providing plant materials.

Funding

This study was funded by grants from the China Agriculture Research System of MOF and MARA (CARS-26), the Henan Province Key R & D and Promotion Projects (212102110119), the Agricultural Science and Technology Innovation Program, and Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2023-ZFRI-03).

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Authors and Affiliations

  1. National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, 450009, People’s Republic of China

    Hailei Huang, Miaomiao Lin, Leiming Sun, Ran Wang, Yukuo Li & Xiujuan Qi

  2. Shiyan Economic crops Research Institute, Shiyan, Hubei, China

    Hailei Huang

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  1. Hailei Huang
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  2. Miaomiao Lin
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  3. Leiming Sun
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  4. Ran Wang
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  5. Yukuo Li
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Contributions

HH: prepared samples, conducted experiments and wrote the original manuscript. ML, LS and RW: gave good suggestions during manuscript preparation. YL and XQ: organized the whole research.

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Correspondence to Yukuo Li or Xiujuan Qi.

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Huang, H., Lin, M., Sun, L. et al. Screening and identification of photoresponse factors in kiwifruit (Actinidia arguta) development. Mol Biol Rep 51, 112 (2024). https://doi.org/10.1007/s11033-023-09073-1

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