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Influential factors for optimizing and strengthening mesenchymal stem cells and hematopoietic stem cells co-culture

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Abstract

Mesenchymal stem cells (MSCs) and Hematopoietic stem cells (HSCs) are two types of bone marrow stem cells that can proliferate and differentiate into different cell lineages. HSCs interact with MSCs under protective conditions, called niche. Numerous studies have indicated supportive effects of MSCs on HSCs proliferation and differentiation. Furthermore, HSCs have many clinical applications and could treat different hematologic and non-hematologic diseases. For this purpose, there is a need to perform in vitro studies to optimize their expansion. Therefore, various methods including co-culture with MSCs are used to address the limitations of HSCs culture. Some parameters that might be effective for improving the MSC/ HSC co-culture systems. Manipulating culture condition to enhance MSC paracrine activity, scaffolds, hypoxia, culture medium additives, and the use of various MSC sources, have been examined in different studies. In this article, we investigated the potential factors for optimizing HSCs/ MSCs co-culture. It might be helpful to apply a suitable approach for providing high-quality HSCs and improving their therapeutic applications.

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Abbreviations

MSCs:

Mesenchymal stem cells

HSCs:

Hematopoietic stem cells

UCB:

Umbilical cord blood

WJ:

Wharton jelly

Flt3L:

FMS-like tyrosine kinase-3 ligand

SCF:

Stem cell factor

G-CSF:

Granulocyte colony-stimulating factor

GM-CSF:

Granulocyte–macrophage colony-stimulating factor

TPO:

Thrombopoietin

Angs:

Angiopoietins

Wnt:

Wingless-related integration

LT-HSCs:

Long-term hematopoietic stem cells

ST-HSCs:

Short-term hematopoietic stem cells

IGFBP1:

Insulin-like growth factor-binding protein-1

NS:

Nucleostemin

Nfix:

Nuclear factor I/X

CFU:

Colony forming unit

CAFC:

Cobblestone area forming cells

DNMT1:

DNA methyl-transferase

LTC-IC:

Long-term culture- initiating cell

EPCR:

Endothelial protein C receptor

VLA-4, 5:

Very late antigen-4, 5

CXCR4:

C-X-C chemokine receptor 4

GVHD:

Graft versus host disease

bFGF:

Basic fibroblast growth factor

LIF:

Leukemia inhibitory factor

SDF:

Stromal-derived factor

TGFβ-RII:

Transforming growth factor-beta receptor II

MVs:

Microvesicles

miRNAs:

MicroRNAs

ROS:

Reactive oxygen species

WJ-MSCs:

Wharton jelly-derived mesenchymal stem cells

ESCs:

Embryonic stem cells

BM-MSCs:

Bone marrow-derived MSCs

CXCL12:

C-X-C chemokine ligand 12

UC:

Umbilical cord

CFU-Meg:

Megakaryocyte colony-forming unit

LFA:

Lymphocyte function-associated antigen

HOXB4:

Homeobox protein B 4

HOXA9:

Homeobox protein A 9

BMI1:

Polycomb complex protein BMI-1

PCL:

Polycaprolactone

FN:

Fibronectin

PDMS:

Polymethyl siloxane

PEG:

Polyethylene glycol

VEGF:

Vascular endothelial growth factor

TEPA:

Tetraethylenepentamine

SR1:

Aryl hydrocarbon receptor antagonist

UM171:

Pyrimidoindole derivatives

P18IN003, P18IN011:

P18 inhibitory molecules in the cell cycle

CHIR99021:

Type of glycogen synthase kinase 3β inhibitor

BIO:

Another kind of glycogen synthase kinase 3β inhibitor

NR-101:

A small non-peptidyl agonist molecule of c-MPL

5azaD / TSA:

5-Aza-2-deoxycytidine / trichostatin A

GAR:

Garcinol; a plant-derived histone acetyltransferase

AhR:

Aryl hydrocarbon receptor

TMEM183A:

Transmembrane protein 183A

PROCR:

Protein C receptor

mTOR:

Mammalian target of rapamycin

PTEN:

Phosphatase and tensin homolog

PI3K:

Phosphoinositide 3-kinase

GSK3β:

Glycogen synthase kinase 3 beta

STAT5:

Signal transducer and activator of transcription 5

HIF-1α:

Hypoxia-inducible factor-1α

HAT:

Histone acetyltransferase

VPA:

Valproic acid

HDAC:

Histone deacetylase

DEAB:

Diethylaminobenzaldehyde

zVADfmk/zLLYfmk:

Caspase and calpain inhibitors

ALDH:

Aldehyde dehydrogenase

5-HT:

5-Hydroxytryptamine

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Acknowledgements

This work was founded by the Hamadan University of Medical Sciences, Code: IR.UMSHA.REC.1400.183, [grant number: 140004012854]. Special thanks to Delaram Yaghoubzadeh.

Funding

This work was founded by the Hamadan University of Medical Sciences [grant No. 140004012854].

Author information

Authors and Affiliations

  1. Central Medical Laboratory, Vice Chancellor for Public Health, Hamadan University of Medical Science, Hamadan, Iran

    Mandana Shirdare

  2. Department of Medical Laboratory Sciences, School of Paramedicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Fatemeh Amiri

  3. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

    Mohammad Pouya Samiee

  4. Student Research Committee, Hamadan University of Medical Science, Hamadan, Iran

    Armita Safari

Authors
  1. Mandana Shirdare
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  2. Fatemeh Amiri
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  3. Mohammad Pouya Samiee
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  4. Armita Safari
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Contributions

Mandana Shirdareh has written the first draft of the manuscript. Mohammad Pouya Samiee and Armita Safari have participated to write the first manuscript and figure preparation. Fatemeh Amiri has reviewed the text. All authors commented on previous versions of the manuscript. All authors reviewed the manuscript.

Corresponding author

Correspondence to Fatemeh Amiri.

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Shirdare, M., Amiri, F., Samiee, M.P. et al. Influential factors for optimizing and strengthening mesenchymal stem cells and hematopoietic stem cells co-culture. Mol Biol Rep 51, 189 (2024). https://doi.org/10.1007/s11033-023-09041-9

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