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Understanding the bacterial community structure associated with the Eichhornia crassipes rootzone

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A Correction to this article was published on 01 February 2024

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Abstract

Background

Plant microbiome acts as an interface between plants and their environment, aiding in the functioning of the ecosystem, such as protection against abiotic and biotic stress along with improving nutrient uptake. The rhizosphere is an essential interface for the interaction between plants and microbes and plays a substantial part in the removal as well as uptake of heavy metals and antibiotics from contaminated locations. Eichhornia crassipes is a promising plant that contains a rich community of microbes in its rhizosphere. Microorganism’s association with plants embodies a crucial pathway via which humans can also be exposed to antibiotic-resistant genes and bacteria.

Methods and results

In our earlier study enhanced removal of ciprofloxacin was observed by plant growth-promoting Microbacterium sp. WHC1 in the presence of E. crassipes root exudates. Therefore, the V3-V4, hypervariable region of the 16 S rRNA gene was studied to assess the bacterial diversity and functional profiles of the microbiota associated with plant roots. Using the QIIME software program, 16 S rRNA data from the Next Generation Sequencing (NGS) platform was examined. Alpha diversity including Chao1, Observed Shannon, and Simpson index denote significantly higher bacterial diversity. Proteobacteria (79%) was the most abundant phylum which was present in the root samples followed by Firmicutes (8%) and Cyanobacteria (8%). Sulfuricurvum (36%) is the most abundant genus belonging to the family Helicobacteraceae and the species kujiense in the genus Sulfuricurvum is the most abundant species present in the root sample. Also, the bacterial communities in the rhizoplane of Eichhornia crassipes harbor the genes conferring resistance to beta-lactams, tetracycline, fluoroquinolones, and penams.

Conclusion

Metagenomic studies on the E. crassipes microbiome showed that the bacterial communities constituting the root exudates of the Eichhornia aid them to survive in a polluted environment.

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Data availability

The Illumina sequencing data has been submitted to NCBI with Bio-project ID: PRJNA926139.

Change history

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Acknowledgements

The authors would like to acknowledge the University of Delhi.

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Authors and Affiliations

  1. Department of Zoology, University of Delhi, Delhi, 110007, India

    Chandra Kant Singh & Dileep Kumar Singh

  2. Department of Zoology, Sri Guru Tegh Bahadur Khalsa College, University of Delhi, Delhi, 110007, India

    Kushneet Kaur Sodhi

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CKS & KKS: conceptualization, experimentation, writing, and editing. DKS: conceptualization and editing.

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Correspondence to Kushneet Kaur Sodhi.

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The original online version of this article was revised: The second affiliation is corrected as 'Department of Zoology, Sri Guru Tegh Bahadur Khalsa College, University of Delhi, Delhi-110007, India.

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Singh, C.K., Sodhi, K.K. & Singh, D.K. Understanding the bacterial community structure associated with the Eichhornia crassipes rootzone. Mol Biol Rep 51, 35 (2024). https://doi.org/10.1007/s11033-023-08979-0

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