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Schizophyllan promotes osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells in vitro

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Abstract

Background

Bioactive polysaccharides are a promising way for bone disease prevention with high efficiency. Schizophyllan (SPG) is a polysaccharide derived from a species of fungus with anticancer, antitumor, and anti-inflammatory effects. In the present study, for the first time, the cell proliferation, osteogenic markers, mineral deposition, and osteogenic gene expression of human adipose tissue-derived mesenchymal stem cells (hADMSCs) grown on SPG were evaluated by in vitro assays.

Methods and results

The cytotoxicity of SPG was measured using the MTT assay and acridine orange staining. Differentiation of hADMSCs was assessed using alkaline phosphatase (ALP) activity test, cellular calcium content assay, and mineralized matrix staining. To this end, Alizarin red S, von Kossa staining, and the expression of bone-specific markers, including ALP, Runx2, and osteonectin, were used by real-time RT-PCR over a 2-week period. According to the results, SPG at 10 µg/ml concentration was determined as the optimal dosage for differentiation studies. The results of osteogenic differentiation tests showed that compared to the control groups in vitro, SPG enhanced the osteogenic markers and mineralization as well as upregulation of the expression of bone specific genes in differentiated hADMSCs during differentiation.

Conclusions

The results revealed that SPG could be applied as effective factor for osteogenic differentiation in the future. The current study provides insights into the hADMSC-based treatment and introduces promising therapeutic material for individuals who suffer from bone defects and injuries.

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Data availability

The main data supporting the results of this study are available within the paper. The raw datasets are available for research purposes from the corresponding author at reasonable request.

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Acknowledgements

We would like to acknowledge the Clinical Research Development Center of Baqiyatallah Hospital for their technical support. Also, we thank Ms. Elahe Eftekhari for her assistance with this project.

Funding

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Authors and Affiliations

  1. Department of Cellular and Molecular Biology, Faculty of Biology, Science and Research Branch of Islamic Azad University, Tehran, Iran

    Saideh Hemati

  2. Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran

    Ashrafalsadat Hatamian-Zarmi

  3. Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Raheleh Halabian

  4. Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran

    Mohsen Ghiasi

  5. Tissue Engineering and Regenerative Medicine Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Ali Salimi

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  1. Saideh Hemati
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Contributions

All authors contributed to the study conception and design. The first draft of the manuscript was written by SH. All authors read and commented on manuscript. Conceptualization: AS; methodology: AZ, SH; analysis and investigation: SH, RH, MG; writing original draft preparation: SH; editing: SH, AS; resources: SH; supervision: AS, AZ.

Corresponding author

Correspondence to Ali Salimi.

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Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors. The ADMSCs were purchased from the cell bank of the Stem Cells Research Center, Tehran, Iran.

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Hemati, S., Hatamian-Zarmi, A., Halabian, R. et al. Schizophyllan promotes osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells in vitro. Mol Biol Rep 50, 10037–10045 (2023). https://doi.org/10.1007/s11033-023-08877-5

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  • DOI: https://doi.org/10.1007/s11033-023-08877-5

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