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Nisin and non-essential amino acids: new perspective in differentiation of neural progenitors from human-induced pluripotent stem cells in vitro

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Abstract

Over the past decades, stem cell therapy has been investigated as a promising approach towards various diseases, including neurodegenerative disorders. Stem cells show the capability to differentiate into neuronal progenitor cells in vitro. In the present study, the differentiation potential of human-induced pluripotent stem cells (hiPSCs) into neural lineages was examined under the efficient induction media containing forskolin and 3-isobutyl-1-methyl-xanthine (IBMX) in the presence of nisin (Ni), non-essential amino acids (NEAA) and combination of those (NEAA-Ni) in vitro. The optimum concentrations of these factors were obtained by MTT assay and acridine orange (AO) staining. The effect of Ni and NEAA on the expression rate of neural-specific markers including NSE, MAP2, and ß-tubulin III was studied via immunocytochemistry (ICC) and real-time RT-PCR analyses. Our results indicated that the induction medium containing Ni or NEAA increased the gene and protein expression of NSE, MAP2, and β-tubulin III on the 14th differentiation day. On the other hand, NEAA-Ni showed a less-differentiated hiPSCs compared to Ni and NEAA alone. In conclusion, the obtained results illustrated that Ni and NEAA could be applied as effective factors for neural differentiation of hiPSCs in the future.

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Abbreviations

AO:

Acridine orange

AA:

Amino acids

cAMP:

Cyclic adenosine monophosphate

ESCs:

Embryonic stem cells

EB:

Ethidium Bromide

hiPSCs:

Human induced pluripotent stem cells

IBMX:

3-Isobutyl-1-methyl-xanthine

MSCs:

Mesenchymal stem cells

MAP-2:

Microtubule-associated protein-2

NSE:

Neuron-specific enolase

Ni:

Nisin

NEAA-Ni:

Ni in combination with NEAA

NEAA:

Non-essential amino acids

RNA:

Ribonucleic acids

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The work was not supported by any Department.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Elahe Eftekhari

  2. Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

    Marzieh Ghollasi

  3. Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Raheleh Halabian

  4. Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

    Maryam Soltanyzadeh

  5. Immunogenetics Research Center, Department of Medical Biotechnology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

    Seyed Ehsan Enderami

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  1. Elahe Eftekhari
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  2. Marzieh Ghollasi
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  5. Seyed Ehsan Enderami
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Correspondence to Marzieh Ghollasi.

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This article does not contain any studies with human participants or animals performed by any of the authors. The hiPSCs were purchased from The Stem Cell Technology Research Center.

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Eftekhari, E., Ghollasi, M., Halabian, R. et al. Nisin and non-essential amino acids: new perspective in differentiation of neural progenitors from human-induced pluripotent stem cells in vitro. Human Cell 34, 1142–1152 (2021). https://doi.org/10.1007/s13577-021-00537-9

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  • DOI: https://doi.org/10.1007/s13577-021-00537-9

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