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The first complete mitochondrial genomes for Serranus papilionaceus and Serranus scriba, and their phylogenetic position within Serranidae

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Abstract

Background

Butterfly-winged comber, Serranus papilionaceus Valenciennes, 1832, was recently resurrected and so it is no longer considered as a junior synonym of the Painted comber, Serranus scriba (Linneus, 1758). This calls for a more comprehensive phylogenetic assessment using mitochondria DNA genomes to better understand the relationship and delineate these two species.

Methods and results

Next-generation Sequencing was applied to sequence the genome of these two Serranus species. The data generated was then used to construct the mitochondrial genome of these two species. This produced the first complete mitochondrial genomes for the genus Serranus here represented by Serranus papilionaceus and Serranus scriba. These two mitochondrial genomes are 16,514 bp and 16,512 bp respectively, and both contained the typical 37 genes found in vertebrates (13 protein-coding genes, two ribosomal RNAs, 22 transfer RNAs), together with the OL and the control region.

Conclusions

These mitochondrial genomes provide a new insight into the phylogenetic and evolutionary connections between the various subfamilies within Serranidae, while providing new molecular data that can be applied to discriminate between the studied species.

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References

  1. Parenti P, Randall JE (2020) An annotated checklist of the fishes of the family Serranidae of the world with description of two new related families of fishes. FishTaxa 15:1–170

    Google Scholar 

  2. Vella A, Vella N (2016) Genetic barcoding and preliminary phylogenetic analysis of Serranidae species from Maltese coastal waters, with a perspective on their Mediterranean phylogeography. Nat Eng Sci 1:66–77

    Google Scholar 

  3. Sadovy YJ (1994) Grouper stocks of the western central Atlantic: the need for management and management needs. Ann Gulf Caribb Fish Inst Proc 43:43–64

    Google Scholar 

  4. Sadovy de Mitcheson Y, Craig MT, Bertoncini AA, Carpenter KE, Cheung WL, Choat JH, Cornish AS, Fennessy ST, Ferreira BP, Heemstra PC, Liu M, Myers RF, Pollard DA, Rhodes KL, Rocha LA, Russell BC, Samoilys MA, Sanciangco J (2013) Fishing groupers towards extinction: a global assessment of threats and extinction risks in a billion dollar fishery. Fish Fish 14:119–136

    Article  Google Scholar 

  5. Giglio VJ, Bender MG, Zapelini C, Ferreira CEL (2017) The end of the line? Rapid depletion of a large-sized grouper through spearfishing in a subtropical marginal reef. Perspect Ecol Conserv 15:115–118

    Google Scholar 

  6. IUCN (2022) The IUCN red list of threatened species, version 2021–3. https://www.iucnredlist.org. Accessed 20 Mar 2022

  7. FAO (2022) Fisheries and aquaculture topics. Online query panels. Topics fact sheets. Fisheries Global Information System (FIGIS). https://www.fao.org/figis/servlet/TabLandArea?tb_ds=Capture&tb_mode=TABLE&tb_act=SELECT&tb_grp=COUNTRY. Accessed 20 Mar 2022

  8. Vella A, Vella N, Acosta-Díaz C (2021) Resurrection of the Butterfly-winged Comber, Serranus papilionaceus Valenciennes, 1832 (Teleostei, Serranidae) and its phylogenetic position within genus Serranus. ZooKeys 1017:111–126

    Article  Google Scholar 

  9. Iwamoto T, Wirtz P (2018) A synopsis of the Eastern and Central Atlantic Combers of the Genus Serranus (Teleostei: Scorpaeniformes: Serranidae). Proc Calif Acad Sci 65:1–39

    Google Scholar 

  10. Horton T, Kroh A, Ahyong S, Bailly N, Boury-Esnault N, Brandão SN, et al (2022) World register of marine species. https://www.marinespecies.org. Accessed 20 Mar 2022

  11. Zhuang X, Ding S, Wang J, Wang Y, Su Y (2009) A set of 16 consensus primer pairs amplifying the complete mitochondrial genomes of orange-spotted grouper (Epinephelus coioides) and Hong Kong grouper (Epinephelus akaara). Mol Ecol Resour 9:1551–1553

    Article  CAS  Google Scholar 

  12. Zhuang X, Qu M, Zhang X, Ding S (2013) A comprehensive description and evolutionary analysis of 22 grouper (Perciformes, Epinephelidae) mitochondrial genomes with emphasis on two novel genome organizations. PLoS ONE 8:e73561

    Article  CAS  Google Scholar 

  13. Vella N, Vella A (2021) Characterization of the complete mitogenome of Haifa grouper, Hyporthodus haifensis (Perciformes: Serranidae), and its phylogenetic position within Epinephelini. Mitochondrial DNA B Resour 6:1287–1289

    Article  Google Scholar 

  14. Du FY, Ye L, Wang XH (2015) Complete mitochondrial genome of the sixbar grouper Epinephelus sexfasciatus (Perciformes: Epinephelidae). Mitochondrial DNA 26:461–462

    Article  CAS  Google Scholar 

  15. Guo M, Huang H, Gao Y (2016) Complete mitochondrial genome of darkfin hind Cephalopholis urodeta (Perciformes, Epinephelidae). Mitochondrial DNA B Resour 1:913–916

    Article  Google Scholar 

  16. Hsiao ST, Chen KS, Tseng CT, Wu CL (2016) Complete mitochondrial genome of the sixblotch hind Cephalopholis sexmaculata (Pisces: Perciformes). Mitochondrial DNA Part A 27:1018–1019

    Article  CAS  Google Scholar 

  17. Li JL, Liu M, Wang YY (2014) Complete mitochondrial genome of the chocolate hind Cephalopholis boenak (Pisces: Perciformes). Mitochondrial DNA 25:167–168

    Article  Google Scholar 

  18. Liu M, Li JL, Ding SX, Liu ZQ (2013) Epinephelus moara: a valid species of the family Epinephelidae (Pisces: Perciformes). J Fish Biol 82:1684–1699

    Article  CAS  Google Scholar 

  19. Meng L, Zhang Y, Gong L, Gao Y (2021) Characterization of the complete mitochondrial genome of Cephalopholis miniata (Perciformes, Serranidae) and its phylogenetic analysis. Mitochondrial DNA B Resour 6:1976–1978

    Article  Google Scholar 

  20. Shen M, Shi X, Qu M, Chen J (2013) Complete mitochondrial genome of squaretail coral grouper Plectropomus areolatus (Perciformes, Epinephelidae). Mitochondrial DNA 24:365–367

    Article  CAS  Google Scholar 

  21. Sun Y, Wei T, Su X, Li T (2016) The complete mitochondrial genome of Grammistes sexlineatus (Perciformes, Serranidae). Mitochondrial DNA Part A 27:821–823

    Article  CAS  Google Scholar 

  22. Wang H, Guo L, Ding S (2016) The complete mitochondrial genome of Diploprion bifasciatum (Perciformes, Serranidae). Mitochondrial DNA Part A 27:3137–3138

    Article  CAS  Google Scholar 

  23. Xie Z, Yu C, Guo L, Li M, Yong Z, Liu X, Meng Z (2016) Ion torrent next-generation sequencing reveals the complete mitochondrial genome of black and reddish morphs of the Coral Trout Plectropomus leopardus. Mitochondrial DNA Part A 27:609–612

    Article  CAS  Google Scholar 

  24. Zhu K, Huang G, Zhang D, Guo Y, Yu D (2016) The complete nucleotide sequence of Malabar grouper (Epinephelus malabaricus) mitochondrial genome. Mitochondrial DNA Part A 27:2087–2088

    CAS  Google Scholar 

  25. Satoh TP, Miya M, Mabuchi K, Nishida M (2016) Structure and variation of the mitochondrial genome of fishes. BMC Genom 17:719

    Article  Google Scholar 

  26. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Mentjies P, Drummond A (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649

    Article  Google Scholar 

  27. Chan PP, Lowe TM (2019) tRNAscan-SE: searching for tRNA genes in genomic sequences. Methods Mol Biol 1962:1–14

    Article  CAS  Google Scholar 

  28. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acids Res 22:4673–4680

    Article  CAS  Google Scholar 

  29. Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    Article  CAS  Google Scholar 

  30. Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9:772

    Article  CAS  Google Scholar 

  31. Vella N, Vella A (2021) Characterization and comparison of the complete mitochondrial genomes of two stingrays, Dasyatis pastinaca and Dasyatis tortonesei (Myliobatiformes: Dasyatidae) from the Mediterranean Sea. Mol Biol Rep 48(2021):219–226

    Article  CAS  Google Scholar 

  32. Craig MT, Hastings PA (2007) A molecular phylogeny of the groupers of the subfamily Epinephelinae (Serranidae) with a revised classification of the Epinephelini. Ichthyol Res 54:1–17

    Article  Google Scholar 

  33. Ceruso M, Mascolo C, Lowe EK, Palma G, Anastasio A, Sordino P, Pepe T (2018) The complete mitochondrial genome of the common pandora Pagellus erythrinus (Perciformes: Sparidae). Mitochondrial DNA B Resour 3:624–625

    Article  Google Scholar 

  34. Mascolo C, Ceruso M, Chirollo C, Palma G, Anastasio A, Sordino P, Pepe T (2019) The complete mitochondrial genome of the Angolan dentex Dentex angolensis (Perciformes: Sparidae). Mitochondrial DNA B Resour 4:1245–1246

    Article  Google Scholar 

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Acknowledgements

The authors would like to thank fishers from Malta and the Canary Islands, who have supported this research and assisted in specimen collection.

Funding

The research disclosed in this publication has been supported by funds through the BioCon_Innovate Research Excellence Grant from the University of Malta awarded to AV and FINS project through the REACH HIGH Scholars Programme-Post Doctoral Grants awarded to NV. The latter is part-financed by the European Union, Operational Programme II—Cohesion Policy 2014—2020 “Investing in human capital to create more opportunities and promote the well-being of society”—European Social Fund.

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Authors and Affiliations

  1. Conservation Biology Research Group, Department of Biology, University of Malta, Msida, Malta

    Adriana Vella & Noel Vella

  2. BICREF - Biological Conservation and Research Foundation, Hamrun, Malta

    Adriana Vella, Noel Vella & Carolina Acosta-Díaz

Authors
  1. Adriana Vella
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  2. Noel Vella
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  3. Carolina Acosta-Díaz
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Contributions

AV and NV contributed towards the design of the research, data analyses and manuscript writing. All authors contributed towards specimen collection and species identification. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Adriana Vella.

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The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

This study did not require ethical approval as it made use of muscle tissue collected from dead specimens that were caught by local fishers and were to be sold at the respective local fish markets.

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Vella, A., Vella, N. & Acosta-Díaz, C. The first complete mitochondrial genomes for Serranus papilionaceus and Serranus scriba, and their phylogenetic position within Serranidae. Mol Biol Rep 49, 6295–6302 (2022). https://doi.org/10.1007/s11033-022-07437-7

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  • DOI: https://doi.org/10.1007/s11033-022-07437-7

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