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Transcriptional profile of cumulus associated GJA1, PTX3, PRSS35, and SERPINE2 genes with oocytes and embryonic development in water buffalo

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Abstract

Background

In the present study, the potential of different groups of cumulus-oocyte complexes (COC’s) for in vitro maturation (IVM) and embryonic development was assessed in two groups of COC’s of water buffalo. Further, the expression pattern of cumulus-associated GJA1, PTX3, PRSS35, and SERPINE2 genes and their effects on embryonic development was analyzed. Slaughterhouse-derived buffalo COC’s were graded into groups A and B. An equal number of 410 COC’s were taken in both groups. IVM was carried out using Slaughterhouse-derived buffalo epididymis. A remarkable degree of cumulus expansion was noticed in group A (92.68%) as compared to group B (81.25%) oocytes. On in vitro fertilization (IVF) and embryo culture, group A produced a significantly higher rate of cleavage and blastocyst (92.682 ± 0.7179% and 42.682 ± 0.9683%) as compared to group B (85.365 ± 0.7608% and 31.707 ± 0.9688%). Also, the transcriptional analysis of cumulus-associated genes revealed significantly higher expression in group A as compared to group B.

Results

It was revealed that oocytes having good cumulus mass had a higher developmental potential. Based on differential gene expression of cumulus-associated genes, different quality of COC’s, and the resultant embryos after IVF, it was concluded that these genes could be used as a marker for predicting the developmental competence of the oocytes.

Conclusion

We concluded that morphologically good quality of COC’s had a higher developmental competence, and also the differential expressions of cumulus-associated genes in cumulus cells and embryos. So, we can conclude that these genes could be used as marker genes for predicting the developmental competence of buffalo’s oocytes.

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Data availability

All data and materials for the study were provided by the Director of Animal Biotechnology Centre, NDVSU Jabalpur, M.P., India.

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Authors and Affiliations

  1. Animal Biotechnology Center, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, 482004, India

    Lalit Mohan Jeena, Dharmendra Kumar, Sandeep Rahangdale, Ajit Pratap Singh & Bikash Chandra Sarkhel

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  1. Lalit Mohan Jeena
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  2. Dharmendra Kumar
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  3. Sandeep Rahangdale
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  4. Ajit Pratap Singh
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  5. Bikash Chandra Sarkhel
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Contributions

LMJ wrote the manuscript. BCS and DK helped in statistical analysis. SR and APS arranged the figures and references as per journal guidelines.

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Correspondence to Lalit Mohan Jeena.

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The authors declare that they have no Conflict of interest.

Ethical approval

There was no need for ethical approval for this study. Slaughterhouse-derived ovaries and epididymis were used for in vitro transcriptional analysis of cumulus-associated genes. No live animals were used.

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Not needed. In the in vitro study, no live animals were used.

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Not needed. In the in vitro study, no live animals were used.

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Mohan Jeena, L., Kumar, D., Rahangdale, S. et al. Transcriptional profile of cumulus associated GJA1, PTX3, PRSS35, and SERPINE2 genes with oocytes and embryonic development in water buffalo. Mol Biol Rep 49, 6285–6293 (2022). https://doi.org/10.1007/s11033-022-07435-9

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  • DOI: https://doi.org/10.1007/s11033-022-07435-9

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