Abstract
Background
The H1N1 influenza virus causes acute respiratory tract infection, and its clinical symptoms are very similar to those of ordinary influenza. The disease develops rapidly. If the flu is not treated, complications such as pneumonia, respiratory failure, and multiple organ damage can occur, resulting in a high fatality rate. Influenza virus mutates rapidly. At present, there is no specific drug for H1N1, so it is an urgent need for clinical care to find new drugs to treat H1N1.
Materials and methods
The polysaccharide derived from Durvillaea Antarctica green algae has a certain antiviral effect. In this study, the results of CCK-8, apoptosis cycle detection, JC-1 and Western blotting proved that Duvira Antarctic polysaccharide (DAPP) has the ability to inhibit H1N1 infection.
Results
CCK-8 test showed that the DAPP with concentration at 32 μg/mL had no toxicity to MDCK cells. In addition, DAPP reduced cell apoptosis by inhibiting the ERK signaling pathway. Meanwhile, DAPP could increase the expression of STAT3 and significantly inhibited proinflammatory cytokines.
Conclusions
In summary, these results suggested that DAPP may be potential with the ability to resist the H1N1 influenza virus.
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Data availability
Data sharing not applicable to this article as no datasets were generated or analysed during the current study.
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Acknowledgements
This work was supported by the Guangdong Natural Science Foundation (2020A1515110648), the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications (2020A03), Technology Planning Project of Guangzhou (202102010202). Open Project of Guangdong Key Laboratory of Marine Materia Medica (LMM2020-7). The Guangzhou Medical University Students’ Science and Technology Innovation Project (2020AEK03, 2021AEK119, 2021AEK122, 2021AEK125 and 2021AEK128).
Funding
This work was supported by the Guangdong Natural Science Foundation (2020A1515110648), the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications (2020A03), Technology Planning Project of Guangzhou (202102010202). Open Project of Guangdong Key Laboratory of Marine Materia Medica (LMM2020-7). The Guangzhou Medical University Students’ Science and Technology Innovation Project (2020AEK03, 2021AEK119, 2021AEK122, 2021AEK125 and 2021AEK128).
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JS, DC and RZ designed the study, analyzed the experimental data and drafted the manuscript. XL and MZ carried out the experiments. BZ and YL refined the manuscript. All authors read and approved the final manuscript.
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Su, J., Chen, D., Zheng, R. et al. Duvira Antarctic polysaccharide inhibited H1N1 influenza virus-induced apoptosis through ROS mediated ERK and STAT-3 signaling pathway. Mol Biol Rep 49, 6225–6233 (2022). https://doi.org/10.1007/s11033-022-07418-w
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DOI: https://doi.org/10.1007/s11033-022-07418-w