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Current technological interventions and applications of CRISPR/Cas for crop improvement

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Abstract

Efficient and innovative breeding strategies are immensely required to meet the global food demand, nutritional security and sustainable agriculture. Genome editing tools have emerged as an effective technology for site-directed genome modification causing the change in gene expression and protein function for the improvement of various important traits in particular the CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein). As the technology evolved with time, advances have been observed like prime editing, base editing, PAMless editing, Drosha based editing with multiple targets having the potential to fulfill the regulatory processes around the world. These recent interventions are highly proficient, cost-efficient, user-friendly, and holds promise for a major revolution in basic and applied plant biology research in the ever-evolving climatic conditions. In the review, we have discussed the most recent technologies and advances for CRISPR/Cas editing in plants.

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Acknowledgements

KMB thank the Director, ICAR-IIRR and DST-SERB ECR/2017/003133 project for providing resources for research work. NDM aknowledge DST-SERB Project ECR/2017/003133 for providing Junior Research Fellowship.

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ICAR-IIRR Core budget and DST-SERB ECR/2017/003133.

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  1. Priya Shah and Nakul D. Magar have contributed equally.

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  1. Tamil Nadu Agricultural University, Tamil Nadu, India

    Priya Shah

  2. ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, Telangana State, 500030, India

    Nakul D. Magar & Kalyani M. Barbadikar

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  1. Priya Shah
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  2. Nakul D. Magar
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PS and NDM drafted the manuscript and contributed equally. KMB criticallysupervised and corrected the review.

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Correspondence to Kalyani M. Barbadikar.

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Shah, P., Magar, N.D. & Barbadikar, K.M. Current technological interventions and applications of CRISPR/Cas for crop improvement. Mol Biol Rep 49, 5751–5770 (2022). https://doi.org/10.1007/s11033-021-06926-5

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