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Molecular detection of gyrA and mexA genes in Pseudomonas aeruginosa

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Abstract

Background

Pseudomonas aeruginosa is one of the types of bacteria that arises resistance toward fluoroquinolos antibiotics remarkably in recent years.

Methods

Fifty P. aeruginosa isolates were isolated from one hundred clinical samples, investigated the antibiogram activity toward eight different groups of antibiotics. Screening about gyrA gene was done by conventional PCR further more qualitative gene expression of mexA gene was done by using Real-time PCR in 22 MDR isolates, furthermore Relative gene expression analysis of gyrA and mexA was done.

Results

The rate of P. aeruginosa isolates was (41.6%) from total clinical samples, the antibiogram test showed high resistance toward Ceftazidime, Ciprofloxacin, Levofloxacin and Gentamicin (100%), while the sensitivity was observed towards colistin (100%). Screening of gyrA that was achieved by PCR technique showed 22 positive isolates. Furthermore, the 22 isolates appeared high expression level of the efflux pump resistance gene mexA and gyrA gene compared with housekeeping gene rspL gene within fold change ranging (0.18–36 and 1–28.84 respectively) with a mean of 18.46 ct and 18.59 (respectively).

Conclusions

All P. aeruginosa isolates were MDR with high level of efflux pump expression of mexA gene as well as gyrA gene.

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Acknowledgements

The authors would like to thank Mustansiriyah University (https://uomustansiriyah.edu.iq/)/Baghdad, Iraq for its support to complete this work.

Funding

This research received no external funding.

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Authors and Affiliations

  1. Oil Products Distribution Company/Baghdad Distribution Authority, Baghdad, Iraq

    Walaa Hussein Abed

  2. College of Science/Mustansiriyah University, Baghdad, Iraq

    Sawsan Mohammed Kareem

Authors
  1. Walaa Hussein Abed
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  2. Sawsan Mohammed Kareem
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Contributions

All authors contributed equally in writing—original draft preparation, All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Sawsan Mohammed Kareem.

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The authors declare no conflict of interest.

Ethical approval

The Ethics Committee of the Mustansiriyah University approved and oversaw this study.

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All patients gave their written informed consents before inclusion.

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Research involving human and animals participants

This study involved the 120 from patients who are suffering from infections of burns and wounds in Baghdad hospitals, Baghdad, Iraq.

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Abed, W.H., Kareem, S.M. Molecular detection of gyrA and mexA genes in Pseudomonas aeruginosa. Mol Biol Rep 48, 7907–7912 (2021). https://doi.org/10.1007/s11033-021-06820-0

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  • DOI: https://doi.org/10.1007/s11033-021-06820-0

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