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Heat stress aggravates oxidative stress, apoptosis, and endoplasmic reticulum stress in the cerebellum of male C57 mice

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Abstract

Background

The current study was set to assess the effect of heat stress exposure on oxidative stress, apoptosis, and endoplasmic reticulum stress markers in the cerebellum of male mice.

Methods

Fifty male C57BL/6 mice were assigned to five groups of (I) control, (II) heat stress (HS)7, (III) HS14, (IV) HS21, and (V) HS42 groups. Animals in the control group were not exposed to HS. Mice in the II-V groups were exposed to HS once a day over 7, 14, 21, and 42 days, respectively. Cerebellar reactive oxygen species (ROS) levels, expression of heat shock protein (HSP)70 and caspase 3 as well as endoplasmic reticulum stress-related proteins (PERK, p-PERK, CHOP, and Full-length ATF-6) expression were determined on the 7th, 14th, 21st, and 42nd days.

Results

ROS levels and HSP70 expression increased following HS on the 14th, 21st, and 42nd days and the 7th, and 14th days with a peak level of expression on the 14th day following HS. HSP70 levels decreased afterward on the 21st and 42nd days compared with the control group. Besides, exposure to HS for 14, 21, and 42 days resulted in a significant increase in the CHOP and p-PERK levels in the cerebellum compared with the control group. Heat exposure also increased protein expression of cleaved caspase 3 and active ATF-6/Full–length ATF-6 on the 21st and 42nd days in the cerebellum compared with the control animals.

Conclusion

These findings indicated that chronic HS augmented oxidative stress, endoplasmic reticulum stress, and apoptosis pathways in the cerebellum of mice.

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Acknowledgements

The authors thank Neurosciences Research Center staff for their support and assistance with this project.

Funding

This research was supported by a grant from the Neurosciences Research Center, Tabriz University of Medical Sciences (Grant Number:63833) to J-M.

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Authors and Affiliations

  1. Neurosciences Research Center, Tabriz University of Medical Sciences, 51666-14756, Tabriz, Iran

    Hajar Oghbaei, Leila Hosseini, Fereshteh Farajdokht, Sepideh Rahigh Aghsan, Alireza Majdi, Saeed Sadigh-Eteghad & Javad Mahmoudi

  2. Deptartment of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran

    Hajar Oghbaei, Leila Hosseini & Fereshteh Farajdokht

  3. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Siamak Sandoghchian Shotorbani

Authors
  1. Hajar Oghbaei
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  2. Leila Hosseini
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  4. Sepideh Rahigh Aghsan
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  8. Javad Mahmoudi
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Contributions

LH and HO performed the experiments. SRA and AM analyzed interpreted the data. FF, JM, and SSE designed the experiments and edited the paper. SS and JM contributed to the interpretation of the data. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Javad Mahmoudi.

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Authors declare no competing interests related to this article.

Ethical approval

All experiments were approved and performed under the guidelines of the Ethics Committee of Animal Research at the Tabriz University of Medical Sciences (IR.TBZMED.VCR.REC.1398.285) and the National Institutes of Health Guide for Care and Use of Laboratory Animals (Publication Number: 85–23, revised 1985).

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Oghbaei, H., Hosseini, L., Farajdokht, F. et al. Heat stress aggravates oxidative stress, apoptosis, and endoplasmic reticulum stress in the cerebellum of male C57 mice. Mol Biol Rep 48, 5881–5887 (2021). https://doi.org/10.1007/s11033-021-06582-9

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  • DOI: https://doi.org/10.1007/s11033-021-06582-9

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