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Urinary exosomal microRNA-96-5p and microRNA-183-5p expression as potential biomarkers of bladder cancer

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Abstract

Because of low sensitivity and specificity of the currently available urine biomarkers of bladder cancer (BC) detection and painful cystoscopy procedure. Our study aimed to evaluate expression of urinary exosomal miR-96-5p and miR-183-5p as probable non-invasive and accurate biomarkers for the diagnosis and follow up of BC. Using quantitative real-time polymerase chain reaction; expression of exosomal microRNA (miR)-96-5p and miR- 183-5p in the urine samples of 51 patients with BC, 21 patients with benign urinary bladder lesions and in 24 normal individuals as control group was done. Our study results showed higher expressions of both miR-96-5p and miR-183-5p in urine of BC patients in comparison with control group (P < 0.001 for each). Receiver operating characteristic curve (ROC) analysis showed that each microRNA had good sensitivity and specificity to differentiate BC from non-BC patients miR-96-5p 80.4% and 91.8% and miR-183-5p 78.4% and 81.6% respectively compared to cytology (37.3% and 100%). In addition, it was obvious that the sensitivity of combined miR-96-5p and miR-183-5p for the diagnosis of BC reached 88.2%% and specificity reached 87.8%, which were higher than each one alone. We also found that expression of miR-96-5p and miR-183-5p with high grade, and pathological stage was significantly increased. After surgery, collected urine samples showed significantly lower expression of miR-96-5p-: P < 0.001; and miR-183-5p: P = 0.002. In conclusion, urine miR-96-5p and miR-183-5p are promising tumor biomarkers of BC diagnosis; particularly, when they combined with each other or with urinary cytology.

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Authors and Affiliations

  1. Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Amal S. El-Shal, Sally M. Shalaby & Walaa Sarhan

  2. Urology Departments, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Safwat E. Abouhashem

  3. Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Eman H. Abd Elbary

  4. Urology Department, Faculty of Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt

    Samir Azazy

  5. Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Nearmeen M. Rashad

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  1. Amal S. El-Shal
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  2. Sally M. Shalaby
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  3. Safwat E. Abouhashem
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  4. Eman H. Abd Elbary
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  5. Samir Azazy
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All the authors shared in: Design of the work, data collection, data analysis and interpretation, drafting and revision of the article.

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Correspondence to Sally M. Shalaby.

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Written consent was obtained from all participants. The study was approved by the research ethical committee of our institutes. The study was done according to The Code of Ethics of the World Medical Association (Declaration of Helsinki) for studies involving humans.

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El-Shal, A.S., Shalaby, S.M., Abouhashem, S.E. et al. Urinary exosomal microRNA-96-5p and microRNA-183-5p expression as potential biomarkers of bladder cancer. Mol Biol Rep 48, 4361–4371 (2021). https://doi.org/10.1007/s11033-021-06451-5

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