Abstract
We previously reported that Numb, a protein localized to clathrin-coated vesicles, regulates the membrane expression of metabotropic glutamate receptor 5 (mGluR5) and is critical to social behaviors. However, the distinct actions of Numb isoforms on mGluR5 have not been investigated. Here, we showed that the expression patterns of Numb-p72 and Numb-p65, two important isoforms of Numb, were distinct in HEK293T cells. Numb-p72, but not Numb-p65, bound to mGluR5α, and enhanced mGluR5 membrane expression by inhibiting its internalization. Our results suggest that a complete structure is required for Numb to bind to mGluR5 and to modulate mGluR5 trafficking.
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This study was funded by the National Natural Science Foundation of China (81702662) and Zhejiang Provincial Foundation of Natural Science of China (LQ17C090001).
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Wang, N., Wang, DD., Hou, X. et al. Different roles of Numb-p72 and Numb-p65 on the trafficking of metabotropic glutamate receptor 5. Mol Biol Rep 48, 595–600 (2021). https://doi.org/10.1007/s11033-020-06103-0
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DOI: https://doi.org/10.1007/s11033-020-06103-0