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Molecular characterization and different expression patterns of the FABP gene family during goat skeletal muscle development

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Abstract

The FABP (adipocyte fatty acid-binding protein) genes play an important role in intracellular fatty acid transport and considered to be candidate genes for fatness traits in domestic animal. In this study, we cloned the cDNA sequences of goat FABP family genes and their expression patterns were detected by semi-quantitative RT-PCR and quantitative real time RT-PCR. Expression analysis showed that goat FABP1 gene was predominantly expressed in liver, kidney and large intestine. While FABP4 was widely expressed in many tissues with a high expression level was observed in the fat, skeletal muscle, stomach and lung. Notably, FABP2 gene was expressed specifically in small intestine. Moreover, goat FABP3 was expressed at 60 day with the highest level, then significantly (p < 0.01) decreased at the 90 day. No significant expression differences were observed in longissimus dorsi muscles among 3 day, 30 day and 60 day. Goat FABP4 was expressed at 3 day with the lowest level, then significantly (p < 0.01) increased to a peak at the 60 day. In addition, a significant relationship between FABP3 mRNA expression levels and intramuscular fat (IMF) content was observed. These results suggest that the FABP3 and FABP4 may be important genes for meat quality and provides useful information for further studies on their roles in skeletal muscle IMF deposit.

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Acknowledgments

This work was supported by the Grants from Scientific Researcher Fund of Sichuan Provincial Education Department (10ZA049), and the national Spark key Program (2012GA810001), and Sichuan Province Science and Technology Support Program (2011NZ0003, 2011NZ0099-36).

Author information

Authors and Affiliations

  1. Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, People’s Republic of China

    Linjie Wang, Li Li, Jing Jiang, Yan Wang, Tao Zhong & Hongping Zhang

  2. Institute of Nanjiang Brown Goat Breeding Science, Nanjiang, Sichuan, People’s Republic of China

    Yu Chen

  3. Southwest University for Nationalities, Chengdu, Sichuan, People’s Republic of China

    Yong Wang

Authors
  1. Linjie Wang
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  2. Li Li
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  3. Jing Jiang
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  4. Yan Wang
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  5. Tao Zhong
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  6. Yu Chen
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  7. Yong Wang
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  8. Hongping Zhang
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Corresponding author

Correspondence to Hongping Zhang.

Additional information

Linjie Wang and Li Li have contributed equally to this work.

Electronic supplementary material

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11033_2014_3759_MOESM1_ESM.rtf

Fig. S1 Multiple amino acid sequence alignments of goat, human, mouse and pig FABP1, FABP2, FABP3 and FABP4 genes. Protein sequences were obtained from GenBank: NP_001434.1 (FABP1-Homo sapiens); NP_001004046.1 (FABP1-Sus scrofa); NP_059095.1 (FABP1-Mus musculus); NP_032006.1 (FABP2-Mus musculus); NP_000125.2 (FABP2-Homo sapiens); NP_001026950.1 (FABP2-Sus scrofa); NP_004093.1 (FABP3-Homo sapiens); NP_034304.1 (FABP3-Mus musculus); NP_001093401.1 (FABP3-Sus scrofa); NP_001433.1 (FABP4-Homo sapiens); NP_077717.1 (FABP4-Mus musculus); NP_001002817.1 (FABP4-Sus scrofa). Conserved amino acids are highlighted. The putative lipocalin and fatty acid binding protein signatures in their central portion underlined. (RTF 157 kb)

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Wang, L., Li, L., Jiang, J. et al. Molecular characterization and different expression patterns of the FABP gene family during goat skeletal muscle development. Mol Biol Rep 42, 201–207 (2015). https://doi.org/10.1007/s11033-014-3759-4

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  • DOI: https://doi.org/10.1007/s11033-014-3759-4

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