Abstract
Maize (Zea mays L.) is the most important food crop in the world, with significant acreage and production across the globe. However, it is affected by low temperatures throughout its growth process, especially during germination. Therefore, it is important to identify more QTLs or genes associated with germination under low-temperature conditions. For the QTL analysis of traits related to low-temperature germination, we used a high-res genetic map of 213 lines of the intermated B73 × Mo17 (IBM) Syn10 doubled haploid (DH) population, which had 6618 bin markers. We detected 28 QTLs of eight phenotypic characteristics associated with low-temperature germination, while they explained the phenotypic contribution rate of 5.4 ~ 13.34%. Additionally, 14 overlapping QTLs produced six QTL clusters on every chromosome, except for 8 and 10. RNA-Seq found six genes related to low-temperature tolerance in these QTLs, while qRT-PCR analysis demonstrated that the expression trends of the Zm00001d045568 gene in the LT_BvsLT_M group and the CK_BvsCK_M group were highly significantly different at all four-time points (P < 0.01), and encoded the RING zinc finger protein. It was located on qRTL9-2 and qRSVI9-1 and is related to the total length and simple vitality index. These results provided potential candidate genes for further gene cloning and improving the low-temperature tolerance of maize.
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Relevant raw data will be freely available to any researcher wishing to use them for non-commercial purposes, without breaching participant confidentiality (Table S7).
Abbreviations
- QTL:
-
Quantitative trait loci
- RNA-seq:
-
RNA sequencing
- qRT-PCR:
-
Quantitative RT PCR
- qRTL :
-
QTL of relative total length
- qRSVI :
-
QTL of relative simple vitality index
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Acknowledgements
We would like to thank Prof. Hongjun Liu of Shandong Agricultural University for providing the plant material.
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This work was supported by “Countless Project” engineering and technology major project of Heilongjiang province (2019ZX16B03-3) and Backbone of Young Talent Scholar Project (to Yu Zhou, 20XG23).
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YZ and ZHW conceived and designed the experiment. HD, LZ, LD, XZ, and XJL prepared materials. HZ and QYX conducted the experiments. QYX, HZ, and HZZ analyzed the data. QYX, XRW, and YHW wrote the manuscript. ML provided experimental materials. YZ and ZHW revised the final version of the paper. All authors approved the final version of the manuscript.
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Xu, Q., Wang, X., Wang, Y. et al. Combined QTL mapping and RNA-Seq pro-filing reveal candidate genes related to low-temperature tolerance in maize. Mol Breeding 42, 33 (2022). https://doi.org/10.1007/s11032-022-01297-6
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DOI: https://doi.org/10.1007/s11032-022-01297-6