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Identification and fine mapping of qPBR10-1, a novel locus controlling panicle blast resistance in Pigm-containing P/TGMS line

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Abstract

Rice blast is one of the most widespread and devastating diseases in rice production. Tremendous success has been achieved in the identification and characterization of genes and quantitative trait loci (QTLs) conferring seedling blast resistance, however, genetic studies on panicle blast resistance have lagged far behind. In this study, two advanced backcross inbred sister lines (MSJ13 and MSJ18) were obtained in the process of introducing Pigm into C134S and showed significant differences in the panicle blast resistance. One F2 population derived from the crossing MSJ13/MSJ18 was used to QTL mapping for panicle blast resistance using genotyping by sequencing (GBS) method. A total of seven QTLs were identified, including a major QTL qPBR10-1 on chromosome 10 that explains 24.21% of phenotypic variance with LOD scores of 6.62. Furthermore, qPBR10-1 was verified using the BC1F2 and BC1F3 population and narrowed to a 60.6-kb region with six candidate genes predicted, including two genes encoding exonuclease family protein, two genes encoding hypothetical protein, and two genes encoding transposon protein. The nucleotide variations and the expression patterns of the candidate genes were identified and analyzed between MSJ13 and MSJ18 through sequence comparison and RT-PCR approach, and results indicated that ORF1 and ORF2 encoding exonuclease family protein might be the causal candidate genes for panicle blast resistance in the qPBR10-1 locus.

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Funding

This work was supported by the National Natural Science Foundation of China (31801342, 31971868), the Natural Science Foundation of Jiangsu Province, China (BK20181216); the Key Studying and Developing Project of Jiangsu Province for Modern Agriculture (BE2020318, BE2019339-3); Rice Industry Technology System of Yangzhou Comprehensive Experimental Station, Yangzhou, Jiangsu Province, China (CARS-01-60); and the Key Studying and Developing Project of Yangzhou for Modern Agriculture (YZ2019035).

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  1. Lixiahe Agricultural Research Institute of Jiangsu Province, Jiangsu Collaborative Innovation Center for Modern Crop Production, Yangzhou, 225009, China

    Yunyu Wu, Ning Xiao, Yuhong Li, Ling Yu, Yue Cai, Cunhong Pan, Xiaoxiang Zhang, Niansheng Huang, Changhai Zhou, Hongjuan Ji, Jianju Liu, Wei Shi, Zichun Chen & Aihong Li

  2. Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, 210095, China

    Yunyu Wu, Ning Xiao, Yuhong Li, Ling Yu, Yue Cai, Cunhong Pan, Xiaoxiang Zhang, Niansheng Huang, Changhai Zhou, Hongjuan Ji, Jianju Liu, Wei Shi, Zichun Chen & Aihong Li

  3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Qiang Gao & Chengzhi Liang

  4. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Yuese Ning

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  1. Yunyu Wu
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  2. Ning Xiao
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Contributions

YW and AL conceived and designed the experiments; NX, YL, QG, YN, LY, YC, CP, XZ, NH, CZ, HJ, JL, WS, and ZC performed the experiments; YN and CL analyzed the data; YW and AL wrote the paper. All the authors read and approved the final manuscript.

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Correspondence to Aihong Li.

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Supplementary Information

Fig. S1

Plant morphology of MSJ13 and MSJ18 at the flowering stage. (PNG 7198 kb)

High Resolution (TIF 14407 kb)

Table S1.

Information of blast populations for pathogenicity assays at seedling stage. (XLSX 11 kb)

Table S2.

Summary of PCR primer sequences. (XLSX 12 kb)

Table S3.

Panicle blast resistance evaluation of lines harboring Pigm gene using mixed isolates of blast by artificial inoculation (years 2013 to 2015). (XLSX 10 kb)

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Wu, Y., Xiao, N., Li, Y. et al. Identification and fine mapping of qPBR10-1, a novel locus controlling panicle blast resistance in Pigm-containing P/TGMS line. Mol Breeding 41, 75 (2021). https://doi.org/10.1007/s11032-021-01268-3

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