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Allele dosage–dependent selection of recessive F3’H allele homozygote altered anthocyanin composition in sweetpotato

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Abstract

To change the major anthocyanin in the storage roots of sweetpotato (Ipomoea batatas) from purplish cyanidin derivatives to reddish pelargonidin derivatives, the dosage of a recessive allele in the F3’H gene locus was enriched by successive crossing and allele dosage estimation. A putative recessive allele of the F3’H gene, named f3’htm, was found in the cultivar Tanegeshimamurasaki and contained a 57-bp deletion causing a premature stop codon. Starting with the parents having a simplex f3’htm allele, plants considered to be homozygous with f3’htm were obtained in the F4 generation. The storage roots and other pigmented tissues of the homozygous plants showed a reddish color, whereas the original parents or F4 plants without homozygous f3’htm were purplish. More than approximately 90% of the anthocyanins in the storage roots of plants with homozygous f3’htm were pelargonidin derivatives. These results suggest that allele dosage–dependent selection is also possible in autohexaploid crops to develop mutants with novel phenotypes. In addition, the obtained plants with homozygous f3’htm can be important materials for breeding sweetpotato cultivars for pelargonidin production.

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Acknowledgments

The authors thank the members of the Technical Support Center (Operations Unit 3) of KARC/NARO for their assistance in crossing and maintaining the sweetpotato plants. The authors are also grateful for the technical assistance provided by Sanae Nagahashi in DNA extraction and by Kumiko Tokudome in the analysis of anthocyanin pigments. The authors are also grateful to Hiroaki Tabuchi for his useful comments on the study.

Author contribution statement

MT and YT conceived the study, prepared the experimental populations, and performed the genotype analyses. MT analyzed the phenotypes of the plants and wrote the manuscript. TS analyzed anthocyanin composition. All authors read and approved the final manuscript.

Funding

This study was funded by the National Agriculture and Food Research Organization, Japan.

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Authors and Affiliations

  1. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization, Yokoichi 6651-2, Miyakonojo, Miyazaki, 885-0091, Japan

    Masaru Tanaka, Tetsufumi Sakai & Yasuhiro Takahata

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  1. Masaru Tanaka
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  2. Tetsufumi Sakai
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  3. Yasuhiro Takahata
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Correspondence to Masaru Tanaka.

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Tanaka, M., Sakai, T. & Takahata, Y. Allele dosage–dependent selection of recessive F3’H allele homozygote altered anthocyanin composition in sweetpotato. Mol Breeding 39, 152 (2019). https://doi.org/10.1007/s11032-019-1062-8

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