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Construction of dense linkage maps “on the fly” using early generation wheat breeding populations

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Abstract

In plant species, construction of framework linkage maps to facilitate quantitative trait loci mapping and molecular breeding has been confined to experimental mapping populations. However, development and evaluation of these populations is detached from breeding efforts for cultivar development. In this study, we demonstrate that dense and reliable linkage maps can be constructed using extant breeding populations derived from a large number of crosses, thus eliminating the need for extraneous population development. Using 565 segregating F1 progeny from 28 four-way cross breeding populations, a linkage map of the hexaploid wheat genome consisting of 3,785 single nucleotide polymorphism (SNP) loci and 22 simple sequence repeat loci was developed. Map estimation was facilitated by application of mapping algorithms for general pedigrees implemented in the software package CRI-MAP. The developed linkage maps showed high rank-order concordance with a SNP consensus map developed from seven mapping studies. Therefore, the linkage mapping methodology presented here represents a resource efficient approach for plant breeding programs that enables development of dense linkage maps “on the fly” to support molecular breeding efforts.

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Acknowledgments

The authors acknowledge support from the US Wheat and Barley Scab Initiative under ARS Agreement No: 59-0200-3-005 to J.L.G.H. and by the South Dakota Agricultural Experimental Station.

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Authors and Affiliations

  1. Department of Plant Science, South Dakota State University, Brookings, SD, USA

    J. T. Eckard & J. L. Gonzalez-Hernandez

  2. USDA, Agricultural Research Service, Fargo, ND, USA

    S. Chao

  3. USDA, Agricultural Research Service, Manhattan, KS, USA

    P. St Amand & G. Bai

Authors
  1. J. T. Eckard
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  2. J. L. Gonzalez-Hernandez
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  3. S. Chao
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  4. P. St Amand
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  5. G. Bai
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Corresponding author

Correspondence to J. L. Gonzalez-Hernandez.

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Eckard, J.T., Gonzalez-Hernandez, J.L., Chao, S. et al. Construction of dense linkage maps “on the fly” using early generation wheat breeding populations. Mol Breeding 34, 1281–1300 (2014). https://doi.org/10.1007/s11032-014-0116-1

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  • DOI: https://doi.org/10.1007/s11032-014-0116-1

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