Skip to main content
SpringerLink
Log in

Fluorescent Nanosensors Based on Colloidal Quantum Dots for the Determination of Reduced Glutathione

  • NANOMETROLOGY
  • Published:
Measurement Techniques Aims and scope

A fluorescent nanosensor based on colloidal quantum dots CdSe/ZnS modified with mercaptoacetic acid to determine reduced glutathione, a non-protein compound that plays an important role in protection against oxidative stress, is developed. Sample preparation protocols that allow determination of reduced glutathione in a wide range of concentrations are presented. Dependence of the fluorescence intensity of the system on the incubation time for a number of concentrations of reduced glutathione was measured. The possibility of using the proposed nanosensor for reliable and sensitive determination of reduced glutathione in the concentration range from 10 to 1000 μM is confirmed. The results can be used for quantitative determination of reduced glutathione in physiological media, which is of considerable interest for medical diagnostics.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.

Similar content being viewed by others

References

  1. L. Syedmoradi, M. Daneshpour, M. lvandipour, et al., “Point of care testing: The impact of nanotechnology,” Biosens. Bioelectr., 87, 373–387 (2017), DOI: https://doi.org/10.1016/j.bios.2016.08.084.

    Article  Google Scholar 

  2. N. Hildebrandt, C. M. Spillmann, W. R. Algar, et al., “Energy transfer with semiconductor quantum dot bioconjugates: a versatile platform for biosensing, energy harvesting, and other developing applications,” Chem. Rev., 117, No. 2, 536–711 (2017), DOI: https://doi.org/10.1021/acs.chemrev.6b00030.

    Article  Google Scholar 

  3. L. I. Kolesnikova, O. A. Vanteeva, N. A. Kurashova, and B. Ya. Vlasov, “Glutathione as an important component of the thiol disulfide system in the pathogenesis of infertility in overweight men,” Vestn. Ros. Akad. Med., 68, No. 7 (2013), DOI: https://doi.org/10.15690/vramn.v68i7.705.

    Article  Google Scholar 

  4. D. M. Townsend, K. D. Tew, and H. Tapiero, “The importance of glutathione in human disease,” Biomed. Pharmacother., 57, No. 3–4, 145–155 (2003), DOI: https://doi.org/10.1021/ja0203925.

    Article  Google Scholar 

  5. L. Flohe, Glutathione (oxidative stress and disease), CRC Press, N.Y (2018).

    Google Scholar 

  6. E. V. Dorozhko and E. I. Korotkova, “Study of antioxidant properties of biologically active sulfur-containing compounds by voltammetric and spectrophotometric methods,” Khim. Farm. Zh., 44, No. 10, 53–56 (2010), DOI: https://doi.org/10.30906/0023-1134-2010-44-10-53-56.

    Article  Google Scholar 

  7. Y. Li, P. Wu, H. Xu, et al., “Anti-aggregation of gold nanoparticle-based colorimetric sensor for glutathione with excellent selectivity and sensitivity,” Analyst, 136, No. 1, 196–200 (2011), DOI: https://doi.org/10.1039/c0an00452a.

    Article  ADS  Google Scholar 

  8. Zhang N., Qu F., Luo H Q. , and Li N. B., “Sensitive and selective detection of biothiols based on target-induced agglomeration of silvernanoclusters,” Biosens. Bioelectr., 42, 214–218 (2013), DOI: /https://doi.org/10.1016/j.bios.2012.10.090.

    Article  Google Scholar 

  9. R. Deng, X. Xie, M. Vendrell, et al., “Intracellular glutathione detection using MnO2-nanosheetmodified upconversion nanoparticles,” J. Am. Chem. Soc., 133, No. 50, 20168–20171 (2011), DOI: https://doi.org/10.1021/ja2100774.

    Article  Google Scholar 

  10. Mi Y., Lei X., Han H., et al., “A sensitive label-free FRET probe for glutathione based on CdSe/ZnS quantum dots and MnO2 nanosheets,” Analyt. Methods, 10, No. 34, 4170–4177 (2018), DOI: https://doi.org/10.1039/C8AY01532E.

    Article  Google Scholar 

  11. J Pan, Z. Zheng, J. Yang, et al., “A novel and sensitive fluorescence sensor for glutathione detection by controlling the surface passivation degree of carbon quantum dots,” Talanta, 166, 1–7 (2017), DOI: 0.1016/j.talanta.2017.01.033.

  12. J. Liu, C. Bao, X. Zhong, et al., “Highly selective detection of glutathione using a quantum-dot-based OFF–ON fluorescent probe,” Chem. Commun., 46, No. 17, 2971–2973 (2010), DOI: https://doi.org/10.1039/B924299F.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. All-Russia Research Institute of Optophysical Measurements (VNIIOFI), Moscow, Russia

    M. K. Alenichev, A. A. Yushina, E. B. Drozhennikova, I. S. Filimonov & A. D. Levin

  2. Pirogov Russian National Research Medical University, Moscow, Russia

    O. A. Baranova & A. V. Chekanov

Authors
  1. M. K. Alenichev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. A. Yushina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. B. Drozhennikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. S. Filimonov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. O. A. Baranova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. V. Chekanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. D. Levin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. K. Alenichev.

Additional information

Translated from Izmeritel’naya Tekhnika, No. 9, pp. 16–21, September, 2019

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Alenichev, M.K., Yushina, A.A., Drozhennikova, E.B. et al. Fluorescent Nanosensors Based on Colloidal Quantum Dots for the Determination of Reduced Glutathione. Meas Tech 62, 784–789 (2019). https://doi.org/10.1007/s11018-019-01695-x

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11018-019-01695-x

Keywords

Search

Navigation