Abstract
Purpose: Mangiferin is a natural free radical scavenging antioxidant that induces excitation of the central nervous system. However, the mechanism of neuroprotective effect of mangiferin on focal cerebral ischemia has not been fully investigated. The aim of this study was to investigate the protective effect of mangiferin on focal cerebral ischemia in mice.
Methods: Middle cerebral artery occlusion (MCAO) was performed to investigate the effect of mangiferin on focal cerebral ischemia. Mice were randomly divided into 5 groups: sham, MCAO, MCAO + 5 mg/kg mangiferin, MCAO + 20 mg/kg mangiferin and MCAO + 5 mg/kg nimodipine. Neurobehavioral scores, brain edema, brain injury scores, relative infarct size and expression of some inflammatory factors in the brain were evaluated. NF-κB pathway was detected by Western blotting and immunofluorescence.
Results: The results showed that mangiferin effectively attenuated MCAO-induced brain injury, including improvement of neurological impairment, reduction of brain edema, and reduction of infarct size. Compared with the MCAO group, mangiferin significantly inhibited MCAO-induced neuroinflammation, which can be proved by reduced expression levels of TNF-α, IL-1β, iNOS and COX-2. In addition, we found that phosphorylation of IκBα was inhibited and the expression of NF-κB p65 in the nucleus was reduced after the addition of mangiferin.
Conclusion: Our study suggested that mangiferin exerts neuroprotective effects on focal cerebral ischemia in mice by regulating the NF-κB signaling pathway. Mangiferin may be an effective treatment for cerebral ischemia and other neurological disorders.
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Data Availability
The data sets used and analyzed in the current study are available from the corresponding authors upon reasonable request.
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The methodology was carried out by Tianpao Hao. Formal analysis and investigations were carried out by Tianpao Hao, Chan Chen, Shen Yang and Yang Zhang. Data was curated by Chan Chen, Shen Yang and Yang Zhang. Writing–original draft preparation was carried out by Tianpao Hao. Writing-review and editing was carried out by Feiyu Liang. All the authors have read and agreed to the published version of the manuscript.
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Hao, T., Chen, C., Yang, S. et al. Mangiferin exerts neuroprotective effects against focal cerebral ischemia in mice by regulating NF-κB signaling pathway. Metab Brain Dis 38, 383–391 (2023). https://doi.org/10.1007/s11011-022-01066-6
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DOI: https://doi.org/10.1007/s11011-022-01066-6