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Anti-inflammation Effects of Oxysophoridine on Cerebral Ischemia–Reperfusion Injury in Mice

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Abstract

Oxysophoridine (OSR) is a bioactive alkaloid extracted from the Sophora alopecuroides Linn. Our aim is to explore the potential anti-inflammation mechanism of OSR in cerebral ischemic injury. Mice were intraperitoneally pretreated with OSR (62.5, 125, and 250 mg/kg) or nimodipine (Nim) (6 mg/kg) for 7 days followed by cerebral ischemia. The inflammatory-related cytokines in cerebral ischemic hemisphere tissue were determined by immunohistochemistry staining, Western blot and enzyme-like immunosorbent assay (ELISA). OSR-treated groups observably suppressed the nuclear factor kappa B (NF-κB), intercellular adhesion molecule-1 (ICAM-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). OSR-treated group (250 mg/kg) markedly reduced the inflammatory-related protein prostaglandin E2 (PGE2), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8). Meanwhile, it dramatically increased the interleukin-10 (IL-10). Our study revealed that OSR protected neurons from ischemia-induced injury in mice by downregulating the proinflammatory cytokines and blocking the NF-κB pathway.

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Acknowledgments

We are grateful to Dr. Margaret for editing and polishing the manuscript. The study was supported by the National Natural Science Foundation of China (Grant No. 309605060) and the Natural Science Foundation of Ningxia (Grant No. NZ11212).

Conflict of Interest

There is no conflict of interest.

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    Authors and Affiliations

    1. Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China

      Yong-Sheng Wang, Peng Zhao, Hong-Bo Wang, Ru Zhou, Juan Du & Jian-Qiang Yu

    2. College of Nursing, Ningxia Medical University, Yinchuan, 750004, China

      Yu-Xiang Li

    3. Ningxia Medical University, Yinchuan, 750004, China

      Yin-Ju Hao

    4. Medical Sci-Tech Research Center, Ningxia Medical University, Yinchuan, 750004, China

      Jie Wang & Shu-Jing Wang

    5. Ningxia Key Lab of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Yinchuan, 750004, China

      Lin Ma & Tao Sun

    6. Ningxia Hui Medicines Collaborative Innovation Center, Yinchuan, 750004, China

      Jian-Qiang Yu

    7. Department of Pharmacology, Ningxia Medical University and Ningxia Hui Medicines Collaborative Innovation Center, Yinchuan, Ningxia, China

      Jian-Qiang Yu

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    Correspondence to Jian-Qiang Yu.

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    Yong-Sheng Wang and Yu-Xiang Li contributed equally to this work.

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    Wang, YS., Li, YX., Zhao, P. et al. Anti-inflammation Effects of Oxysophoridine on Cerebral Ischemia–Reperfusion Injury in Mice. Inflammation 38, 2259–2268 (2015). https://doi.org/10.1007/s10753-015-0211-4

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