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Irisin alters d-galactose-induced apoptosis by increasing caveolin-1 expression in C2C12 myoblasts and skeletal muscle fibroblasts

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Abstract

Muscle atrophy and skeletal muscle fibrosis are significant pathological manifestations of primary sarcopenia. The regulation of C2C12 myoblast and skeletal muscle fibroblast apoptosis is associated with these pathological changes. Previous studies have indicated that irisin, the cleaved form of fibronectin type III domain-containing protein 5 (FNDC5), can alleviate primary sarcopenia. However, the mechanisms of the effect of irisin in age-related apoptosis remain unknown. Our present research aimed to explore the effect of irisin and the underlying mechanism of d-galactose (d-gal)-induced apoptosis in skeletal muscle fibroblasts and C2C12 myoblasts. We found the opposite effects of d-gal on C2C12 myoblasts and fibroblasts. We also found that irisin suppressed C2C12 cell apoptosis and promoted fibroblast apoptosis. Mechanistically, irisin altered d-gal-induced apoptosis by increasing caveolin-1 expression. Taken together, these findings further demonstrated that irisin is a potential agent that can treat aged-relative muscle atrophy and fibrosis.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on request.

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Funding

This work was financially supported by funding from the National Natural Science Foundation of China (No. 81901424) and the Natural Science Foundation of Chongqing (cstc2021jcyj-msxmX0327).

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Authors and Affiliations

  1. Geriatric Diseases Institute of Chengdu, Department of Geriatrics, Chengdu Fifth People’s Hospital (The Second Clinical Medical College, Affiliated Fifth People’s Hospital of Chengdu University of Traditional Chinese Medicine), Chengdu, 611137, China

    Yaoxuan Wu & Yongxue Yang

  2. Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, 1St You Yi Road, Yu Zhong District, Chongqing, 400010, China

    Yaoxuan Wu, Yongxin Wu, Jing Yu, Yingxiao Zhang, Jinliang Chen, Yue Sun, Kexiang Zhao & Qian Xiao

  3. Department of General Practice, Yongchuan Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing, 400010, China

    Xin Dai

  4. Department of Endocrinology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310001, China

    Jinliang Chen

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  1. Yaoxuan Wu
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Contributions

Conceptualization, Y.W., Y.W. and K.Z.; Data curation, Y.W., Y.W. and J.Y.; Formal analysis, Y.W. and Y.Z; Funding acquisition, J.C. and Y.S.; Investigation, X.D.; Methodology, J.Y.; Project administration, Q.X.; Software, Y.W.; validation, K.Z. and Y.Y.; Supervision, K.Z. and Y.Y.; Visualization, Y.W.; Writing—original draft, Y.W.; Writing—review and editing, Y.W. All authors have read and agreed to the published version of the manuscript. Figure 6 was illustrated using BioRender.

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Correspondence to Yongxue Yang or Kexiang Zhao.

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Wu, Y., Wu, Y., Yu, J. et al. Irisin alters d-galactose-induced apoptosis by increasing caveolin-1 expression in C2C12 myoblasts and skeletal muscle fibroblasts. Mol Cell Biochem (2024). https://doi.org/10.1007/s11010-024-04990-6

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