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Vitamin D improves vascular function and decreases monoamine oxidase A expression in experimental diabetes

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Abstract

The active form of vitamin D, 1,25-dihydroxycholecalciferol (1,25(OH)2D3), was reported to improve vascular function in patients with diabetes, yet the underlying mechanisms remain to be fully elucidated. Monoamine oxidase (MAO), a mitochondrial enzyme, with two isoforms (A and B) that generates hydrogen peroxide (H2O2) as by-product, has been recently reported to contribute to the pathogenesis of endothelial dysfunction in diabetes. The present study assessed the interaction between vitamin D and MAO in the vascular wall in the setting of type 1 experimental diabetes. To this aim, diabetes was induced in male Wistar rats via a single injection of streptozotocin (STZ, 50 mg/kg, IP) and 1 month later thoracic aortas were harvested and used for organ bath studies and H2O2 measurements. MAO expression was assessed by immunohistochemistry and RT-PCR. Endothelial function was evaluated in isolated aortic rings in the absence vs. presence of 1,25(OH)2D3 (100 nM, 24 h incubation). In diabetic animals, we found a significant reduction in the endothelial-dependent relaxation to acetylcholine and an increased expression of the MAO-A isoform, respectively. Vitamin D significantly improved vascular function, mitigated oxidative stress and decreased MAO-A expression in diabetic vascular preparations. In conclusion, MAO-A is induced in diabetic aortas and vitamin D can improve diabetes-induced endothelial dysfunction by modulating the MAO-A expression.

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Acknowledgements

The study was funded by the institutional grant of “Victor Babeş” University of Medicine and Pharmacy Timișoara code PIII-C5-PCFI-2017/2018-01.

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  1. Adrian Sturza and Adrian Văduva have contributed equally to the work.

Authors and Affiliations

  1. Department of Functional Sciences - Pathophysiology, “Victor Babeş” University of Medicine and Pharmacy, Timişoara, 2, Eftimie Murgu Sq., 300041, Timișoara, Romania

    Adrian Sturza, Diana Uțu, Corina Rațiu, Oana Duicu & Danina M. Muntean

  2. Center for Translational Research and Systems Medicine, “Victor Babeş” University of Medicine and Pharmacy, Timişoara, Romania

    Adrian Sturza, Oana Duicu & Danina M. Muntean

  3. Department of Morphopathology, “Victor Babeş” University of Medicine and Pharmacy, Timişoara, Romania

    Adrian Văduva

  4. Department of Surgery I, “Victor Babeş” University of Medicine and Pharmacy, Timişoara, Romania

    Norbert Pop & Sorin Olariu

  5. Department of Pediatric Surgery, “Victor Babeş” University of Medicine and Pharmacy, Timişoara, Romania

    Călin Popoiu & Eugen Boia

  6. Department of Anatomy, “Victor Babeş” University of Medicine and Pharmacy, Timişoara, Romania

    Petru Matusz

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  1. Adrian Sturza
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Correspondence to Danina M. Muntean.

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Sturza, A., Văduva, A., Uțu, D. et al. Vitamin D improves vascular function and decreases monoamine oxidase A expression in experimental diabetes. Mol Cell Biochem 453, 33–40 (2019). https://doi.org/10.1007/s11010-018-3429-2

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