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Modulation of PBMC-decay accelerating factor (PBMC-DAF) and cytokines in rheumatoid arthritis

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Abstract

Studies have suggested that abnormal expression of complement regulatory proteins and cytokines contribute significantly to the path-physiology of rheumatoid arthritis. In this context, Decay accelerating factor (DAF) a complement regulatory protein is gaining increased attention. With the notion that immune effecter mechanisms are all interlinked and circulating peripheral blood mononuclear cells (PBMCs) should have a role in a systemic disease like rheumatoid arthritis, we studied the modulation and significance of PBMC-DAF and cytokines in RA. Seventy-five RA patients and 75 healthy controls were recruited. Expression of DAF and cytokines (IFN-γ, IL-17A and IL-10) in the PBMCs of patients and controls was determined. Correlations among DAF, cytokines, and disease activity were evaluated by standard statistical methods. The effect of IFN-γ, IL-17A, and IL-10 on the expression of DAF in patients and controls was studied in vitro. Expression of PBMC-DAF declined in patients both at mRNA and surface level and correlated negatively with the disease activity. Expression of IFN-γ also declined in patients but correlated positively with DAF and negatively with disease activity. Expression of IL-17A and IL-10 was higher in patients. The levels correlated positively with disease activity and negatively with DAF both in patients and controls. In vitro studies indicated that IFN-γ up-regulated DAF expression in PBMCs, whereas IL-17A and IL-10 had negative effect on the same. The decline in the PBMC-DAF is a contributing factor in manifestations of RA. Cytokine environment contributes to this decline. These findings brought novel insights into the complement-cytokine axis in the path-physiology of RA.

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Acknowledgments

The authors are grateful to University Grants Commission (UGC), New Delhi, for financial support. We are thankful to all the volunteers of the patient and control groups for their participation in this study.

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Authors and Affiliations

  1. Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India

    Roma Pahwa & Nibhriti Das

  2. Department of Medicine, All India Institute of Medical Sciences, New Delhi, India

    Uma Kumar

  3. Lab Medicine & Biochemistry, Nayati Multi super speciality Hospital, Mathura, 281003, U.P, India

    Nibhriti Das

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  1. Roma Pahwa
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Pahwa, R., Kumar, U. & Das, N. Modulation of PBMC-decay accelerating factor (PBMC-DAF) and cytokines in rheumatoid arthritis. Mol Cell Biochem 414, 85–94 (2016). https://doi.org/10.1007/s11010-016-2661-x

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