Skip to main content
SpringerLink
Log in

Studies on the Production of Broad Spectrum Antimicrobial Compound Polypeptide (Actinomycins) and Lipopeptide (Fengycin) from Streptomyces sp. K-R1 Associated with Root of Abutilon indicum against Multidrug Resistant Human Pathogens

  • Published:
International Journal of Peptide Research and Therapeutics Aims and scope Submit manuscript

Abstract

Endophytic actinomycetes associated with medicinal plants of Chhattisgarh are rich source of novel antimicrobial compounds. The aim of this study was to evaluate the production of antibiotics by endophytic Streptomyces sp. K-R1 associated with root of Abutilon indicum against multidrug resistant human pathogens. The 16S rRNA sequence of the isolate K-R1 has been identified as Streptomyces parvulus NBRC 13193T (AB184326) with the GenBank database (accession number KY777591). Streptomyces parvulus K-R1 grown under submerged fermentation condition optimized by central composite design (glucose—11.25 g/l, soybean meal 12.17 g/l, calcium carbonate—1.47 g/l, sodium chloride—11.25 g/l; pH 7.18 at 30.51 °C with 4% seed inoculum for 10 days of incubation) exhibited highest activity against multidrug resistant Staphylococcus aureus JNMC-3, Staphylococcus epidermidis JNMC-4, Klebsiella pneumoniae MTCC-3384, Klebsiella pneumoniae JNMC-6, Pseudomonas aeruginosa MTCC-741, Proteus vulgaris JNMC-7, Candida albicans MTCC-183 and Aspergillus niger MTCC-872. The structures of the active compounds were elucidated by UV–Vis spectroscopy, 1H and 13C NMR, FTIR and ESIMS. Actinomycin D, actinomycin X0β and fengycin were detected in crude extracts and major components were eluted by HPLC at 10.63, 10.88 and 6.3 min respectively. In this case, a high yield of antibiotics was achieved with Streptomyces parvulus K-R1 fermented in glucose soybean meal broth media with a production of 240 mg/l after 5 days, 290 mg/l after 8 days and 340 mg/l after 11 days, which can be used in industrial fermentation process to obtain high yields. To the author’s best knowledge, it is the first report of fengycin production by Streptomyces parvulus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Balagurunathan R, Subramanian A (1993) Studies on marine Streptomyces nigrifaciens taxonomy and standardization of antibiotic production. Cienc Mar 19:435–443

    Article  CAS  Google Scholar 

  • Castillo UF, Strobel GA, Ford EJ, Hess WM, Porter H, Jensen JB, Albert H, Robison R (2002) Munumbicins, wide-spectrum antibiotics produced by Streptomyces NRRL 30562, endophytic on Kennedia nigriscans. Microbiology 148:2675–2685

    Article  CAS  PubMed  Google Scholar 

  • Castillo U, Harper JK, Strobel GA, Sears J, Alesi K, Ford E, Lin J, Hunter M, Maranta M, Ge H, Yaver D, Jensen JB, Porter H, Robison R, Miller D, Hess WM, Condron M, Teplow D (2003) Kakadumycins, novel antibiotics from Streptomyces sp. NRRL 30566, an endophyte of Grevillea pteridifolia. FEMS Microbiol Lett 234:183–190

    Article  CAS  Google Scholar 

  • Chandrakar S, Gupta A (2017) Antibiotic potential of endophytic actinomycetes of medicinal herbs against human pathogenic bacteria. Proc Natl Acad Sci India B 87:905–915

    CAS  Google Scholar 

  • Chen C, Song F, Wang Q, Abdel-Mageed WM, Guo H, Fu C, Hou W, Dai H, Liu X, Yang N, Xie F, Yu K, Chen R, Zhang L (2012) A marine-derived Streptomyces sp. MS449 produces high yield of actinomycin X2 and actinomycin D with potent anti-tuberculosis activity. Appl Microbiol Biotech. https://doi.org/10.1007/s00253-012-4079-z

    Article  Google Scholar 

  • Ezra D, Castillo UF, Strobel GA, Hess WM, Porter H, Jensen JB, Condron MAM, Teplow DB, Sears J, Maranta M, Hunter M, Weber B, Yaver D (2004) Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp. Microbiology 150:785–793

    Article  CAS  PubMed  Google Scholar 

  • Gallo M, Katz E (1972) Regulation of secondary metabolite biosynthesis catabolic repression of phenoxazinone synthase and actinomycin formation by glucose. J Bacteriol 109:659–667

    CAS  PubMed  PubMed Central  Google Scholar 

  • Golinska P, Wypij M, Agarkar G, Rathod D, Dahm H, Rai M (2015) Endophytic actinobacteria of medicinal plants: diversity and bioactivity. Anton Leeuw 108:267–289

    Article  Google Scholar 

  • Guan S, Suttler I, Lin W, Guo D, Grabley S (2005) p-Aminoacetophenonic acids produced by a mangrove endophyte: Streptomyces griseus sub sp. J Nat Prod 68:1198–1200

    Article  CAS  PubMed  Google Scholar 

  • Hamza AA, Ali HA, Clark BR, Murphy CD, Elobaid EA (2013) Isolation and characterization of actinomycin D producing Streptomyces sp. from Sudanese soil. Afr J Biotechnol 12:2624–2632

    CAS  Google Scholar 

  • Igarashi Y, Ogura H, Furihata K, Oku N, Indananda C, Thamchaipenet A (2011) Maklamicin, an antibacterial polyketide from an endophytic Micromonospora sp. J Nat Prod 74:670–674

    Article  CAS  PubMed  Google Scholar 

  • Indanada C, Igarashi Y, Ikeda M, Oikawa T, Thamchaipenet A (2013) Linfuranone A, a new polyketide from plant derived Microbispora sp. GMKU 363. J Antibiot 66:675–677

    Article  CAS  Google Scholar 

  • Jeon YS, Lee K, Park SC, Kim BS, Cho YJ, Ha SM (2014) EzEditor: a versatile sequence alignment editor for both rRNA-and protein-coding genes. Int J Syst Evol Microbiol 64:689–691

    Article  CAS  PubMed  Google Scholar 

  • Jha SS, Joshi SJ, Geetha SJ (2016) Lipopeptide production by Bacillus subtilis R1 and its possible applications. Braz J Microbiol 47:955–964

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Katz E, Pienta P, Sivak A (1956) The role of nutrition in the synthesis of actinomycin. Appl Microbiol 6:236–241

    Google Scholar 

  • Kawato M, Shinobu R (1959) Cover slip culture of Streptomyces herbaricolour nov. sp. supplement: a simple technique for the microscopical observation. Mem Osaka Univ Lib Arts Educ 8:114–119

    Google Scholar 

  • Khieu TN, Liu MJ, Nimaichand S, Quach NT, Ky SC, Ph QT, Vu TT, Nguyen TD, Xiong Z, Prabhu DM, Li WJ (2015) Characterization and evaluation of antimicrobial and cytotoxic effects of Streptomyces sp. HUST012 isolated from medicinal plant Dracaena cochinchinensis Lour. Front Microbiol. https://doi.org/10.3389/fmicb.2015.00574

    Article  PubMed  PubMed Central  Google Scholar 

  • Kim N, Shin JC, Kim W, Hwang BY, Kim BS, Hong YS, Lee D (2006) Cytotoxic 6 alkylsalicylic acids from the endophytic Streptomyces laceyi. J Antibiot 59:797–800

    Article  CAS  Google Scholar 

  • Kulkarni M, Gorthi S, Banerjee G, Chattopadhyay P (2017) Production, characterization and optimization of actinomycin D from Streptomyces hydrogenans IB310, a (n antagonistic bacterium against phytopathogens). Biocatal Agric Biotechnol 10:69–74

    Article  Google Scholar 

  • Kurosawa K, Bui VP, Van EJL, Willis LB, Lessard PA, Ghiviriga I, Sambandan TG, Rha CK, Sinskey AJ (2005) Characterization of Streptomyces MITKK-103, a newly isolated actinomycin X2 producer. Appl Microbiol Biotechnol 72:145–154

    Article  CAS  PubMed  Google Scholar 

  • Lee JH, Kim YG, Lee K, Kim CJ, Park DJ, Ju Y, Lee JC, Wood TK, Lee J (2016) Streptomyces-derived actinomycin D inhibits biofilm formation by Staphylococcus aureus and its hemolytic activity. Biofouling 32:45–56

    Article  CAS  PubMed  Google Scholar 

  • Meienhofer J, Atherton E (1973) Structure activity relationship in the actinomycins. Adv Appl Microbiol 16:203–300

    Article  CAS  PubMed  Google Scholar 

  • Munimbazi C, Bullerman LB (1998) Isolation and partial characterization of antifungal metabolites of Bacillus pumilus. J Appl Microbiol 84:959–968

    Article  CAS  PubMed  Google Scholar 

  • Nafis A, Oubaha B, Elhidar N, Ortlieb N, Kulik A, Niedermeyer T, Hassani L, Barakate M (2018) Novel production of two new non polyenic antifungal macrolide derivatives by Streptomyces Z26 isolated from Moroccan rhizospheric soil. Online J Biol Sci. https://doi.org/10.3844/ojbsci.2018

    Article  Google Scholar 

  • Praveen V, Tripathi CKM (2009) Studies on the production of actinomycin-D by Streptomyces griseoruber: a novel source. Lett Appl Microbiol 49:450–455

    Article  CAS  PubMed  Google Scholar 

  • Praveen V, Tripathi CKM, Bihari V, Srivastava SC (2008) Production of actinomycin D by the mutant of a new isolate of Streptomyces sindenensis. Braz J Microbiol 39:689–692

    Article  PubMed  PubMed Central  Google Scholar 

  • Pridham TG, Gottlieb D (1948) The utilization of carbon compounds by some Actinomycetales as an aid for species determination. J Bacteriol 56:107–114

    CAS  PubMed  PubMed Central  Google Scholar 

  • Rahman MA, Islam MZ, Khondkar P, Islam MA (2010) Characterization and antimicrobial activities of a polypeptide antibiotic isolated from a new strain of Streptomyces parvulus. Bangla Pharma J 13:14–17

    Google Scholar 

  • Roy S, Banerjee D (2015) Broad spectrum antibacterial activity of granaticinic acid, isolated from Streptomyces thermoviolaceus NT1; an endophyte in Catharanthus roseus (L.) G. Don. J Appl Pharma Sci 5:6–11

    Article  CAS  Google Scholar 

  • Salam N, Khieu TN, Liu MJ, Vu TT, Ky SC, Quach NT, Phi QT, Narsing Rao MP, Fontana A, Sarter S, Li WJ (2017) Endophytic actinobacteria associated with Dracaena cochinchinensis Lour: isolation, diversity and their cytotoxic activities. BioMed Res Int. https://doi.org/10.1155/2017/1308563

    Article  PubMed  PubMed Central  Google Scholar 

  • Shetty PR, Buddana SK, Tatipamula VB, Naga VVV, Ahmad J (2014) Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity. Braz J Microbiol 45:303–312

    Article  PubMed  PubMed Central  Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  • Solanki R, Kundu A, Das P, Khanna M (2015) Characterization of antimicrobial compounds from Streptomyces sp. World J Pharma Res 4:1626–1641

    Google Scholar 

  • Sousa MFVQ, Lopes CE, Junior NP (2001) A chemically defined medium for production of actinomycin D by Streptomyces parvulus. Braz Arch Biol Technol 44:227–231

    Article  Google Scholar 

  • Sousa MFVQ, Lopes CE, Pereira NJ (2002) Development of a bioprocess for the production of actinomycin D. Braz J Chem Eng 19:277–285

    Article  CAS  Google Scholar 

  • Taechowisan T, Wanbanjob A, Tuntiwachwuttikul P, Taylor WC (2006) Identification of Streptomyces sp. Tc022, an endophyte in Alpinia galanga, and the isolation of actinomycin D. Ann Microbiol 56:113–117

    Article  CAS  Google Scholar 

  • Taechowisan T, Chanaphat S, Ruensamran W, Phutdhawong SW (2014) Antibacterial activity of new flavonoids from Streptomyces sp. BT01; an endophyte in Boesenbergia rotunda (L) Mansf. J Appl Pharma Sci 4:8–13

    CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Usha R, Ananthaselvi P, Venil CK, Palaniswamy M (2010) Antimicrobial and antiangiogenesis activity of Streptomyces parvulus KUAP106 from mangrove soil. Eur J Biol Sci 2:77–83

    Google Scholar 

  • Wang F, Xu M, Li Q, Sattler I, Lin W (2010) P-amino acetophenonic acids produced by a mangrove endophyte Streptomyces sp. (strain HK10552). Molecules 15:2782–2790

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wei YH, Wang LC, Chen WC, Chen SY (2010) Production and characterization of fengycin by indigenous Bacillus subtilis F29-3 originating from a potato farm. Int J Mol Sci 11:4526–4538

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wei Z, Xu C, Wang J, Lu F, Bie X, Lu Z (2017) Identification and characterization of Streptomyces flavogriseus NJ-4 as a novel producer of actinomycin D and holomycin. Peer J. https://doi.org/10.7717/peerj.3601

    Article  PubMed  PubMed Central  Google Scholar 

  • Williams WK, Katz E (1977) Development of a chemically defined medium for the synthesis of actinomycin-D by Streptomyces parvulus. Antimicrob Agents Chemother 11:281–290

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang X, Ye X, Chai W, Lian XY, Zhang Z (2016) New metabolites and bioactive Actinomycins from marine derived Streptomyces sp. ZZ338. Marine Drugs 14:181–189

    Article  CAS  PubMed Central  Google Scholar 

Download references

Acknowledgements

Authors are thankful to the Head, SLS, PRSU, Raipur for providing the necessary facilities for the research. One of the authors (SC) is grateful to University Grant Commission, New Delhi, India for financial support in the form of Basic Science Research (F.7-145/2007BSR), SRF. Financial support from DST under FIST program and UGC for DRS-SAP III are gratefully acknowledged. The authors also thank Microbial Type Culture Collection and Gene Bank (MTCC), Chandigarh for providing cultures and Pandit Jawaharlal Nehru Medical College, Raipur, Chhattisgarh for providing clinical cultures for research.

Funding

This study was funded by University Grant Commission, New Delhi, India (F.7-145/2007BSR).

Author information

Authors and Affiliations

  1. Microbiology Research Laboratory, School of Studies in Life Science, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, 492010, India

    Sandhya Chandrakar & Ashwini Kumar Gupta

Authors
  1. Sandhya Chandrakar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ashwini Kumar Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sandhya Chandrakar.

Ethics declarations

Conflict of interest

Authors declares that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 1097 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chandrakar, S., Gupta, A.K. Studies on the Production of Broad Spectrum Antimicrobial Compound Polypeptide (Actinomycins) and Lipopeptide (Fengycin) from Streptomyces sp. K-R1 Associated with Root of Abutilon indicum against Multidrug Resistant Human Pathogens. Int J Pept Res Ther 25, 779–798 (2019). https://doi.org/10.1007/s10989-018-9727-4

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10989-018-9727-4

Keywords

Search

Navigation