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Immune Response of the VEGF/bFGF Complex Peptide Vaccine and Function of Immune Antibodies in Inhibiting Migration of HUVEC Cells and Proliferation of Cancer Cells

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Abstract

Overexpression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) plays a key role in tumor angiogenesis and metastasis in tumors. VEGF/bFGF complex peptide (VBP3) was designed to elicit the body to produce both high titer anti-VEGF and anti-bFGF antibodies to inhibit tumor angiogenesis and tumor growth. BALB/c mice were immunized with the VEGF/bFGF complex peptide, and the immune responses were assayed. Splenocytes were separated from the immunized mice and the CD4, CD8 T cells and IFN-γ were assayed by Flow cytometry. The results showed that the VBP3 could effectively stimulate immune response in mice and resulted in the increase of CD4 and CD8 T cells. CD4+ T cells and CD8+ T cells were increased from 10.78 to 15.13 and 6.82 to 11.58 % respectively. Polyclonal antibodies purified from the VBP3 immunized mice showed good anti-proliferation function to lung cancer cells, and resulted in the decrease of phosphroylation level of Akt and Erk assayed by the Western-blot. Transwell assays showed that the migration of HUVEC cells was inhibited by the antibodies. The results revealed that the VBP3 have good immunogenicity and may be used as a vaccine for tumor therapy.

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Acknowledgments

This project was supported by Grants from the National Natural Science Foundation of China (Grant No. 81171446) and the Fundamental Research Funds for the Central Universities (21612112). We thank Yuying Zhou (Institue of Biomedicine in Jinan University) for providing of FCM assay.

Conflict of interest

Lei Pan, Ruiqiang Weng, Jinxia Zhang, Jinsheng Wang, Yong Tang and Ning Deng declare that they have no conflict of interest.

Statement of human and animal rights

All institutional and national guidelines for the care and use of laboratory animals were followed. And this article does not contain any studies with human subjects.

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Authors and Affiliations

  1. Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou, 510632, People’s Republic of China

    Lei Pan, Ruiqiang Weng, Jinxia Zhang, Jinsheng Wang, Yong Tang & Ning Deng

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  1. Lei Pan
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  2. Ruiqiang Weng
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  3. Jinxia Zhang
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  4. Jinsheng Wang
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  5. Yong Tang
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  6. Ning Deng
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Correspondence to Yong Tang or Ning Deng.

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Pan, L., Weng, R., Zhang, J. et al. Immune Response of the VEGF/bFGF Complex Peptide Vaccine and Function of Immune Antibodies in Inhibiting Migration of HUVEC Cells and Proliferation of Cancer Cells. Int J Pept Res Ther 20, 565–574 (2014). https://doi.org/10.1007/s10989-014-9414-z

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  • DOI: https://doi.org/10.1007/s10989-014-9414-z

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