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Chemical and in vitro characterization of epoxidized natural rubber blends for biomedical applications

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Abstract

Cellprene® biomaterial is reported as an angiogenic resorbable elastomeric blend, but it has poor miscibility between polymers. Poly(isoprene) present in Cellprene® is the main component of Natural Rubber Latex, which is extracted from Hevea brasilienses trees. The epoxidation reaction of Natural Rubber not only reduces the number of double bonds but also increases the hydrophilicity of rubber. The mixing of epoxidized Poly(isoprene) with Poly (Lactic-co-Glycolic acid) is an alternative route for the preparation of miscible and biocompatible blends that can be used in Tissue Engineering. The blends developed in this work were obtained by the solvent casting method. The physico-chemical and mechanical properties of the membranes were evaluated and compared with Cellprene® showing a higher miscibility of the blend that were previously epoxidized demonstrated by the approach of the involved Tg value. Biological essays showed non-cytotoxic results with a cell viability greater than 95%. The in vitro interaction of HMV-II cells with the epoxidized blend showed specific epithelial and morphological markers after 24 h of culture. The results indicate the high viability of the epoxidized mixture for application in Tissue Engineering.

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Acknowledgements

This work was supported by grants the CAPES (Brazil) Program. The authors greatly acknowledge CMM-UFRGS for the use of microscopy facilities and Molecular and Protein Analyzes Department of the Hospital de Clínicas de Porto Alegre.

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Authors and Affiliations

  1. Laboratory of Biomaterials & Advanced Ceramics, Federal University of Rio Grande do Sul, Porto Alegre, CP, 91501-970, Brazil

    Nayrim Brizuela Guerra, Júlia Bünecker Cassel & Luis Alberto Loureiro dos Santos

  2. Embryology and Cell Differentiation Laboratory, Clinics Hospital the Porto Alegre, Porto Alegre, Brazil

    Nicole Andréa Corbellini Henckes, Fernanda dos Santos de Oliveira & Elizabeth Obino Cirne-Lima

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  1. Nayrim Brizuela Guerra
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  2. Júlia Bünecker Cassel
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  3. Nicole Andréa Corbellini Henckes
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  6. Luis Alberto Loureiro dos Santos
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Correspondence to Nayrim Brizuela Guerra.

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Guerra, N.B., Cassel, J.B., Henckes, N.A.C. et al. Chemical and in vitro characterization of epoxidized natural rubber blends for biomedical applications. J Polym Res 25, 172 (2018). https://doi.org/10.1007/s10965-018-1542-2

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  • DOI: https://doi.org/10.1007/s10965-018-1542-2

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