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The Modification of Dissolution Kinetics and Solubility of Lysozyme Crystals by Ionic Liquids

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Abstract

Knowing about the dissolution kinetics and solubility is necessary for controlling crystallization separation process of lysozyme. In this study, the impact of four ionic liquids (ILs), namely, 1-butyl-3-methylimidazolium tetrafluoroborate [C4mim]BF4, 1-butyl-3-methylimidazole chloride [C4mim]Cl, 1-butyl-3-methylimidazole bromide [C4mim]Br, and 1,3-dimethylimidazolium iodide [dmim]I, on the dissolution rate of lysozyme was determined in an aqueous solution at 20 ℃, pH 5.01, under 101.3 kPa. The results revealed that the dissolution rate of lysozyme increased with increasing concentrations of [C4mim]BF4 and [C4mim]Cl, while it remained stable with increasing concentrations of [C4mim]Br. In contrast, the dissolution rate gradually decreased with increasing concentrations of [dmim]I. This suggests that the interaction between lysozyme molecules is influenced by the ILs, leading to variations in the dissolution rates. Additionally, the effect of anions and cations on the equilibrium solubility was analyzed. The results indicated that the order of anionic and cationic effects on equilibrium solubility is as follows: BF4− < Cl− < Br− = [C4mim]+  < [dmim]+  < I−. Furthermore, dissolution kinetic models were established, which could be used to predict the dissolution behavior of large molecules like lysozyme in ILs aqueous solution. These findings have significant implications for the design of crystallization process and optimization of parameters during lysozyme recovery.

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Acknowledgements

This work was funded by the National Natural Science Foundation of China (21978206) and Tianjin Health Research Project (ZC20112).

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  1. Xuanhe Zhu and Chengzhi Wei have contributed equally to this work.

Authors and Affiliations

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China

    Xuanhe Zhu, Na Li & Zhanzhong Wang

  2. Tianjin Medical Union Center, Tianjin, 300121, China

    Chengzhi Wei

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  1. Xuanhe Zhu
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  2. Chengzhi Wei
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Contributions

XZ contributed to conceptualization, methodology, analysis, investigation, writing – original draft, and writing-review & editing. CW contributed to writing-review & modifying and analysis. NL contributed to conceptualization, writing-review & editing, and visualization. ZW contributed to conceptualization, writing-review & editing, resources, supervision, and project administration.

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Correspondence to Zhanzhong Wang.

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Zhu, X., Wei, C., Li, N. et al. The Modification of Dissolution Kinetics and Solubility of Lysozyme Crystals by Ionic Liquids. J Solution Chem (2024). https://doi.org/10.1007/s10953-023-01344-6

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