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Characterization of Physicochemical and Antibacterial Properties of Gelatin and Inulin Nanobiocomposite Films Containing Crystalline Nanocellulose and Malva sylvestris Extract

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A Correction to this article was published on 30 March 2022

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Abstract

This study aimed to develop and characterize gelatin and inulin nanobiocomposite films with crystalline nanocellulose (CNC) and Malva sylvestris extract (MSE) for an active packaging system application. Fourier transform infrared structural conformations approved the formation of interactions between gelatin matrix, inulin, and other additives. According to the morphology study, the addition of inulin and CNC resulted in forming a dense and compact structure. Moreover, the addition of CNC enhanced the film samples’ thermal properties and crystalline structure. The compensated for inulin’s detrimental effect on mechanical parameters. The gelatin film sample containing CNC, MSE, and 50% inulin exhibited the least water vapor permeability, moisture content, and highest contact angle. The inclusion of CNC and inulin increased the L* value of the film samples significantly (p 0.05), which was reduced by incorporating MSE. Additionally, MSE-containing gelatin-based films inhibited Listeria monocytogenes, Staphylococcus aureus, and Salmonella enteritis. In conclusion, the combination of gelatin and inulin and the addition of CNC and MSE resulted in gelatin-based films with suitable physicochemical properties and antibacterial activity. Nanocomposite films developed in this study can be employed as an active packaging system for various foodstuffs.

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References

  1. Hernoaez-Cortez C, Palma-Martinez I, Gonzalez-Avila LU et al (2017) Food poisoning caused by bacteria (food toxins). Poison Specif Toxic Agents to Nov Rapid Simpl Tech Anal 33:1

    Google Scholar 

  2. Sherafatkhah Azari S, Alizadeh A, Asefi N, Hamishehkar H (2021) Investigation the effect of ZnO and β-glucan on chemical and microbial characteristic of gelatin based biodegradable film over storage of chicken fillet. Food Sci Technol 18:169–180

    Google Scholar 

  3. Mousavi Kalajahi SE, Alizadeh A, Hamishehkar H et al (2021) Orange juice processing waste as a biopolymer base for biodegradable film formation reinforced with cellulose nanofiber and activated with nettle essential oil. J Polym Environ. https://doi.org/10.1007/s10924-021-02195-2

    Article  Google Scholar 

  4. Noorbakhsh-Soltani SM, Zerafat MM, Sabbaghi S (2018) A comparative study of gelatin and starch-based nano-composite films modified by nano-cellulose and chitosan for food packaging applications. Carbohydr Polym 189:48–55

    Article  CAS  PubMed  Google Scholar 

  5. Soofi M, Alizadeh A, Hamishehkar H et al (2021) Preparation of nanobiocomposite film based on lemon waste containing cellulose nanofiber and savory essential oil: A new biodegradable active packaging system. Int J Biol Macromol 169:352–361. https://doi.org/10.1016/j.ijbiomac.2020.12.114

    Article  CAS  PubMed  Google Scholar 

  6. Pereda M, Ponce AG, Marcovich NE et al (2011) Chitosan-gelatin composites and bi-layer films with potential antimicrobial activity. Food Hydrocoll 25:1372–1381

    Article  CAS  Google Scholar 

  7. Karimi N, Alizadeh A, Almasi H, Hanifian S (2020) Preparation and characterization of whey protein isolate/polydextrose-based nanocomposite film incorporated with cellulose nanofiber and L. plantarum: a new probiotic active packaging system. LWT. https://doi.org/10.1016/j.lwt.2019.108978

  8. Sahraee S, Milani JM, Ghanbarzadeh B, Hamishehkar H (2017) Physicochemical and antifungal properties of bio-nanocomposite film based on gelatin-chitin nanoparticles. Int J Biol Macromol 97:373–381

    Article  CAS  PubMed  Google Scholar 

  9. Amjadi S, Emaminia S, Nazari M et al (2019) Application of reinforced ZnO nanoparticle-incorporated gelatin bionanocomposite film with chitosan nanofiber for packaging of chicken fillet and cheese as food models. Food Bioprocess Technol 12:1205–1219. https://doi.org/10.1007/s11947-019-02286-y

    Article  CAS  Google Scholar 

  10. Ramos M, Valdes A, Beltran A, GarrigC (2016) Gelatin-based films and coatings for food packaging applications. Coatings 6:41

    Article  Google Scholar 

  11. Amjadi S, Emaminia S, Heyat Davudian S et al (2019) Preparation and characterization of gelatin-based nanocomposite containing chitosan nanofiber and ZnO nanoparticles. Carbohydr Polym 216:376–384. https://doi.org/10.1016/j.carbpol.2019.03.062

    Article  CAS  PubMed  Google Scholar 

  12. Shen Y, Ni ZJ, Thakur K et al (2021) Preparation and characterization of clove essential oil loaded nanoemulsion and pickering emulsion activated pullulan-gelatin based edible film. Int J Biol Macromol 181:528–539. https://doi.org/10.1016/j.ijbiomac.2021.03.133

    Article  CAS  PubMed  Google Scholar 

  13. Huebner J, Wehling RL, Parkhurst A, Hutkins RW (2008) Effect of processing conditions on the prebiotic activity of commercial prebiotics. Int Dairy J 18:287–293

    Article  CAS  Google Scholar 

  14. Cao TL, Yang SY, Song K, Bin (2018) Development of burdock root inulin/chitosan blend films containing oregano and thyme essential oils. Int J Mol Sci 19:1–12. https://doi.org/10.3390/ijms19010131

    Article  CAS  Google Scholar 

  15. Alizadeh A, Aghayi N, Soofi M, Roufegarinejad L (2021) Development of synbiotic added sucrose-free mango nectar as a potential substrate for Lactobacillus casei: Physicochemical characterisation and consumer acceptability during storage. Acta Aliment 50:299–309

    Article  CAS  Google Scholar 

  16. Akhgari A, Farahmand F, Garekani HA et al (2006) Permeability and swelling studies on free films containing inulin in combination with different polymethacrylates aimed for colonic drug delivery. Eur J Pharm Sci 28:307–314

    Article  CAS  PubMed  Google Scholar 

  17. Zabihollahi N, Alizadeh A, Almasi H et al (2020) Development and characterization of carboxymethyl cellulose based probiotic nanocomposite film containing cellulose nanofiber and inulin for chicken fillet shelf life extension. Int J Biol Macromol 160:409

    Article  CAS  PubMed  Google Scholar 

  18. Temiz NN, ֺdemir KS (2021) Microbiological and physicochemical quality of strawberries (Fragaria נananassa) coated with Lactobacillus rhamnosus and inulin enriched gelatin films. Postharvest Biol Technol. https://doi.org/10.1016/j.postharvbio.2020.111433

    Article  Google Scholar 

  19. Orozco-Parra J, MejM, Villa CC (2020) Development of a bioactive synbiotic edible film based on cassava starch, inulin, and Lactobacillus casei. Food Hydrocoll. https://doi.org/10.1016/j.foodhyd.2020.105754

    Article  Google Scholar 

  20. Chaichi M, Hashemi M, Badii F, Mohammadi A (2017) Preparation and characterization of a novel bionanocomposite edible film based on pectin and crystalline nanocellulose. Carbohydr Polym 157:167–175. https://doi.org/10.1016/j.carbpol.2016.09.062

    Article  CAS  PubMed  Google Scholar 

  21. Huq T, Fraschini C, Khan A et al (2017) Alginate based nanocomposite for microencapsulation of probiotic: Effect of cellulose nanocrystal (CNC) and lecithin. Carbohydr Polym 168:61–69. https://doi.org/10.1016/j.carbpol.2017.03.032

    Article  CAS  PubMed  Google Scholar 

  22. Azeredo HMC, Rosa MF, Mattoso LHC (2017) Nanocellulose in bio-based food packaging applications. Ind Crops Prod 97:664–671. https://doi.org/10.1016/j.indcrop.2016.03.013

    Article  CAS  Google Scholar 

  23. Azeredo HMC, Miranda KWE, Rosa MF et al (2012) Edible films from alginate-acerola puree reinforced with cellulose whiskers. LWT-Food Sci Technol 46:294–297

    Article  CAS  Google Scholar 

  24. George J, Kumar R, Sajeevkumar VA et al (2014) Hybrid HPMC nanocomposites containing bacterial cellulose nanocrystals and silver nanoparticles. Carbohydr Polym 105:285–292

    Article  CAS  PubMed  Google Scholar 

  25. Khan A, Khan RA, Salmieri S et al (2012) Mechanical and barrier properties of nanocrystalline cellulose reinforced chitosan based nanocomposite films. Carbohydr Polym 90:1601–1608

    Article  CAS  PubMed  Google Scholar 

  26. Alexieva IN, Baeva MR, Popova AT et al (2021) Edible coatings enriched with Malva sylvestris L. extract. IOP Conf Ser Mater Sci Eng. https://doi.org/10.1088/1757-899X/1031/1/012113

    Article  Google Scholar 

  27. Tabaraki R, Ali Asadi Gharneh H (2012) Chemical Composition and Antioxidant Properties of Malva Sylvestris L. urnal Res Agric Sci 8:59–68

    Google Scholar 

  28. Samavati V, Manoochehrizade A (2013) Polysaccharide extraction from Malva sylvestris and its anti-oxidant activity. Int J Biol Macromol 60:427–436

    Article  CAS  PubMed  Google Scholar 

  29. Cecotti R, Bergomi P, Carpana E, Tava A (2016) Chemical characterization of the volatiles of leaves and flowers from cultivated malva sylvestris var. mauritiana and their antimicrobial activity against the aetiological agents of the european and American foulbrood of honeybees (apis mellifera). Nat Prod Commun 11:1527–1530. https://doi.org/10.1177/1934578x1601101026

    Article  CAS  PubMed  Google Scholar 

  30. Echegaray M, Mondragon G, Martin L et al (2016) Physicochemical and mechanical properties of gelatin reinforced with nanocellulose and montmorillonite. J Renew Mater 4:206–214. https://doi.org/10.7569/JRM.2016.634106

    Article  CAS  Google Scholar 

  31. Nassiri R, Mohammady Nafchi A (2013) Antimicrobial and barrier properties of bovine gelatin films reinforced by nano TiO2. J Chem Heal Risks 3:21–28

    Google Scholar 

  32. Neus Angles M, Dufresne A (2000) Plasticized starch/tuniein whiskers nanocomposites. 1. Structural analysis. Macromolecules 33:8344–8353. https://doi.org/10.1021/ma0008701

    Article  Google Scholar 

  33. Fathi N, Almasi H, Pirouzifard MK (2018) Effect of ultraviolet radiation on morphological and physicochemical properties of sesame protein isolate based edible films. Food Hydrocoll 85:136–143. https://doi.org/10.1016/j.foodhyd.2018.07.018

    Article  CAS  Google Scholar 

  34. Amjadi S, Almasi H, Pourfathi B, Ranjbaryan S (2021) Gelatin films activated by cinnamon essential oil and reinforced with 1D, 2D and 3D nanomaterials: physical and release controlling properties. J Polym Environ. https://doi.org/10.1007/s10924-021-02097-3

    Article  Google Scholar 

  35. Chenwei C, Zhipeng T, Yarui M et al (2018) Physicochemical, microstructural, antioxidant and antimicrobial properties of active packaging films based on poly(vinyl alcohol)/clay nanocomposite incorporated with tea polyphenols. Prog Org Coatings 123:176–184. https://doi.org/10.1016/j.porgcoat.2018.07.001

    Article  CAS  Google Scholar 

  36. Leite LSF, Ferreira CM, Correa AC et al (2020) Scaled-up production of gelatin-cellulose nanocrystal bionanocomposite films by continuous casting. Carbohydr Polym. https://doi.org/10.1016/j.carbpol.2020.116198

    Article  PubMed  Google Scholar 

  37. George J, Siddaramaiah (2012) High performance edible nanocomposite films containing bacterial cellulose nanocrystals. Carbohydr Polym 87:2031–2037. https://doi.org/10.1016/j.carbpol.2011.10.019

    Article  CAS  Google Scholar 

  38. Li K, Jin S, Chen H, Li J (2019) Bioinspired interface engineering of gelatin/cellulose nanofibrils nanocomposites with high mechanical performance and antibacterial properties for active packaging. Compos Part B Eng 171:222–234. https://doi.org/10.1016/j.compositesb.2019.04.043

    Article  CAS  Google Scholar 

  39. Mondragon G, Peodriguez C, Gonzalez A et al (2015) Bionanocomposites based on gelatin matrix and nanocellulose. Eur Polym J 62:1–9

    Article  CAS  Google Scholar 

  40. Li K, Jin S, Chen H et al (2017) A high-performance Soy protein isolate-based nanocomposite film modified with microcrystalline cellulose and Cu and Zn nanoclusters. Polym (Basel). https://doi.org/10.3390/polym9050167

    Article  Google Scholar 

  41. Lima HLS, Gon硬ves C, Cerqueira M et al (2018) Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment. Cellulose 25:6011–6029. https://doi.org/10.1007/s10570-018-1983-0

    Article  CAS  Google Scholar 

  42. Fakhreddin Hosseini S, Rezaei M, Zandi M, Ghavi FF (2013) Preparation and functional properties of fish gelatin-chitosan blend edible films. Food Chem 136:1490–1495. https://doi.org/10.1016/j.foodchem.2012.09.081

    Article  CAS  PubMed  Google Scholar 

  43. Pelissari FM, Andrade-Mahecha MM, Amaral Sobral PJ, Menegalli FC (2017) Nanocomposites based on banana starch reinforced with cellulose nanofibers isolated from banana peels. J Colloid Interface Sci 505:154–167

    Article  CAS  PubMed  Google Scholar 

  44. Shabanpour B, Kazemi M, Ojagh SM, Pourashouri P (2018) Bacterial cellulose nanofibers as reinforce in edible fish myofibrillar protein nanocomposite films. Int J Biol Macromol 117:742–751

    Article  CAS  PubMed  Google Scholar 

  45. Hosseini SF, Rezaei M, Zandi M, Farahmandghavi F (2015) Fabrication of bio-nanocomposite films based on fish gelatin reinforced with chitosan nanoparticles. Food Hydrocoll 44:172–182

    Article  CAS  Google Scholar 

  46. He Q, Zhang Y, Cai X, Wang S (2016) Fabrication of gelatin-TiO2 nanocomposite film and its structural, antibacterial and physical properties. Int J Biol Macromol 84:153–160. https://doi.org/10.1016/j.ijbiomac.2015.12.012

    Article  CAS  PubMed  Google Scholar 

  47. Amjadi S, Nazari M, Alizadeh SA, Hamishehkar H (2020) Multifunctional betanin nanoliposomes-incorporated gelatin/chitosan nanofiber/ZnO nanoparticles nanocomposite film for fresh beef preservation. Meat Sci. https://doi.org/10.1016/j.meatsci.2020.108161

    Article  PubMed  Google Scholar 

  48. Azari SS, Alizadeh A, Roufegarinejad L et al (2021) Preparation and characterization of gelatin/β-glucan nanocomposite film incorporated with ZnO nanoparticles as an active food packaging system. J Polym Environ 29:1143–1152

    Article  Google Scholar 

  49. Soukoulis C, Behboudi-Jobbehdar S, Yonekura L et al (2014) Stability of Lactobacillus rhamnosus GG in prebiotic edible films. Food Chem 159:302–308. https://doi.org/10.1016/j.foodchem.2014.03.008

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. Tibolla H, Pelissari FM, Martins JT et al (2019) Banana starch nanocomposite with cellulose nanofibers isolated from banana peel by enzymatic treatment: in vitro cytotoxicity assessment. Carbohydr Polym 207:169–179. https://doi.org/10.1016/j.carbpol.2018.11.079

    Article  CAS  PubMed  Google Scholar 

  51. Almasian A, Najafi F, Eftekhari M et al (2020) Polyurethane/carboxymethylcellulose nanofibers containing Malva sylvestris extract for healing diabetic wounds: preparation, characterization, in vitro and in vivo studies. Mater Sci Eng C. https://doi.org/10.1016/j.msec.2020.111039

    Article  Google Scholar 

  52. Niu X, Liu Y, Song Y et al (2018) Rosin modified cellulose nanofiber as a reinforcing and co-antimicrobial agents in polylactic acid/chitosan composite film for food packaging. Carbohydr Polym 183:102–109. https://doi.org/10.1016/j.carbpol.2017.11.079

    Article  CAS  PubMed  Google Scholar 

  53. Tsai YH, Yang YN, Ho YC et al (2018) Drug release and antioxidant/antibacterial activities of silymarin-zein nanoparticle/bacterial cellulose nanofiber composite films. Carbohydr Polym 180:286–296. https://doi.org/10.1016/j.carbpol.2017.09.100

    Article  CAS  PubMed  Google Scholar 

  54. Alizadeh-Sani M, Khezerlou A, Ehsani A (2018) Fabrication and characterization of the bionanocomposite film based on whey protein biopolymer loaded with TiO2 nanoparticles, cellulose nanofibers and rosemary essential oil. Ind Crops Prod 124:300–315. https://doi.org/10.1016/j.indcrop.2018.08.001

    Article  CAS  Google Scholar 

  55. Amjadi S, Almasi H, Ghadertaj A, Mehryar L (2020) Whey protein isolate-based films incorporated with nanoemulsions of orange peel (Citrus sinensis) essential oil. Prep Charact. https://doi.org/10.1111/jfpp.15196

    Article  Google Scholar 

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Acknowledgements

The authors gratefully thank the supports of the Islamic Azad University, Tabriz Branch, Tabriz, Iran. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Correspondence to Ainaz Alizadeh.

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Nikoukheslat, H.D., Alizadeh, A., Roufegarinejad, L. et al. Characterization of Physicochemical and Antibacterial Properties of Gelatin and Inulin Nanobiocomposite Films Containing Crystalline Nanocellulose and Malva sylvestris Extract. J Polym Environ 30, 3078–3090 (2022). https://doi.org/10.1007/s10924-022-02398-1

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