Skip to main content
Log in

Development of Eco-friendly Probiotic Edible Coatings Based on Chitosan, Alginate and Carboxymethyl Cellulose for Improving the Shelf Life of UF Soft Cheese

  • Original Paper
  • Published:
Journal of Polymers and the Environment Aims and scope Submit manuscript

Abstract

The objective of the current work was to evaluate the efficiency of novel antimicrobial edible coatings based on chitosan (CH), sodium alginate (SA), and carboxymethyl cellulose (CMC) with an eco-friendly antimicrobial microcrystalline cellulose (AMCC) and probiotic strains (Bifidobacterium lactis, Lactobacillus acidophilus, and Lactobacillus casei). These edible films were applied for preservatives UF soft cheese for 45 days, instead of using traditional plastic sheet. The probiotic edible films were evaluated by using SEM, FT-IR tools. Additionally, the mechanical properties, water vapor permeability, water solubility, and antimicrobial properties were examined. The fashioned probiotic edible films had an antimicrobial effect against all tested microbes, the high microbial effect was observed for edible films with CH and SA. Furthermore, the coated cheese was evaluated chemically, microbiologically, and sensory during 45 days. The dry matter, protein, and fat contents of cheese treatments coated with SA and CMC decreased during the storage period. Additionally, the probiotic counts in all films were more than 8.0 log CFU/g after 45 days. It was also observed that the edible films containing SA and CH can preserve cheese from spoilage. Correspondingly, the sensory scores were indicated the overall acceptability of cheese in this study was SA, CMC, and CH followed by control. Therefore, the formulation of the antimicrobial edible coating can be applied for soft cheese to control the spoilage and maintain the stability of products.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Al-Tayyar NA, Youssef AM, Al-hindi R (2020) Edible coatings and antimicrobial nanoemulsions for enhancing shelf life and reducing foodborne pathogens of fruits and vegetables: a review. Sustain Mater Technol 26:e00215

    Google Scholar 

  2. Salehi F (2019) Characterization of new biodegradable edible films and coatings based on seeds gum: a review. J Packag Technol Res 3:1–9

    Google Scholar 

  3. Cerqueira MA, Lima AM, Souza BWS, Teixeira JA, Moreira RA, Vicente AA (2009) Functional polysaccharides as edible coatings for cheese. J Agric Food Chem 57(4):1456–1462

    CAS  PubMed  Google Scholar 

  4. Cerqueira MA, Sousa-Gallagher MJ, Macedo I, Rodriguez-Aguilera R, Souza BWS, Teixeira JA, Vicente AA (2010) Use of galactomannan edible coating application and storage temperature for prolonging shelf-life of “regional” cheese. J Food Eng 97(1):87–94

    CAS  Google Scholar 

  5. Rodriguez-Turienzo L, Cobos A, Diaz O (2012) Effects of edible coatings based on ultrasound-treated whey proteins in quality attributes of frozen Atlantic salmon (Salmosalar). Innov Food Sci Emerg Technol 14:92–98

    CAS  Google Scholar 

  6. Youssef AM, El-Sayed SM (2018) Bionanocomposites materials for food packaging. applications: concepts and future outlook. Carbohyd Polym 193:19–27

    CAS  Google Scholar 

  7. Soukoulis C, Behboudi-Jobbehdar S, Macnaughtan W, Parmenter C, Fisk ID (2017) Stability of Lactobacillus rhamnosus GG incorporated in edible films: impact of anionic biopolymers and whey protein concentrate. Food Hydrocolloids 70:345–355

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Salehi F (2020) Effect of coatings made by new hydrocolloids on the oil uptake during deep- fat frying: a review. J Food Process Preserv. https://doi.org/10.1111/jfpp.14879

    Article  Google Scholar 

  9. Salehi F (2020) Edible coating of fruits and vegetables using natural gums: a review. Int J Fruit Sci. https://doi.org/10.1080/15538362.2020.1746730

    Article  Google Scholar 

  10. Nair MS, Tomar M, Punia S, Kukula-Koch W, Kumar M (2020) Enhancing the functionality of chitosan-and alginate-based active edible coatings/films for the preservation of fruits and vegetables: a review. Int J Biol Macromol 164:304–320

    CAS  PubMed  Google Scholar 

  11. Nawar GAM, Ahmed MM, El-Zawawy W (2008) Method for preparing new nontoxic antibacterial cellulose derivative. Egyptian patent no. 030422.

  12. El-Sayed HS, El-Sayed SM, Nawwar GA (2017) Utilization of novel nontoxic antimicrobial microcrystalline cellulose in manufacture of stirred yoghurt. Der PharmaChemica 9(8):71–79

    CAS  Google Scholar 

  13. Nawar GAM, Abdel Zaher KS, El-khabiry S, El-Ebiary NMA (2020) Utilizing semi-natural antibacterial cellulose to prepare safe azo disperse dyes and their application in textile printing. Fibers Polym 21(6):1293–1299

    Google Scholar 

  14. Al-Tayyar NA, Youssef AM, Al-Hindi R (2020) Antimicrobial food packaging based on sustainable bio-based materials for reducing foodborne pathogens: a review. Food Chem 310:125915

    CAS  PubMed  Google Scholar 

  15. Youssef AM, Assem FM, El-Sayed HS, El-Sayed SM, Elaaser M, Abd El-Salam MH (2020) Synthesis and evaluation of eco-friendly carboxymethyl cellulose/polyvinyl alcohol/CuO bionanocomposites and their use in coating processed cheese. RSC Adv 10:37857

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Wang LZ, Liu L, Holmes J, Kerry JF, Kerry JP (2007) Assessment of film forming potential and properties of protein and polysaccharide-based biopolymer films. Int J Food Sci Technol 42(9):1128–1138

    CAS  Google Scholar 

  17. El-Sayed SM, Ibrahim OA, Kholif AM (2020) Characterization of novel Ras cheese supplemented with jalapeno red pepper. J Food Process Preserv 44:e14535

    CAS  Google Scholar 

  18. El-Sayed SM, Youssef AM (2019) Potential application of herbs and spices and their effects in functional dairy products. Heliyon 5:e01989

    PubMed  PubMed Central  Google Scholar 

  19. Fajardo P, Martins JT, Fuciños C, Pastrana L, Teixeira JA, Vicente AA (2010) Evaluation of a chitosan-based edible film as carrier of natamycin to improve the storability of Saloio cheese. J Food Eng 101(4):349–356

    CAS  Google Scholar 

  20. Al-Tayyar NA, Youssef AM, Al-Hindi RR (2020) Antimicrobial packaging efficiency of ZnO–SiO2 nanocomposites infused into PVA/CS film for enhancing the shelf life of food products. Food Packag Shelf Life 25:100523

    Google Scholar 

  21. Delcenserie V, Taminiau B, Delhalle L, Nezer C, Doyen P, Crevecoeur S, Daube G (2014) Microbiota characterization of a Belgian protected designation of origin cheese, Herve cheese, using metagenomic analysis. J Dairy Sci 97(10):6046–6056

    CAS  PubMed  Google Scholar 

  22. Youssef AM, El-Sayed SM, El-Sayed HS, Salama HH, Assem FM, Abd El-Salam MH (2018) Novel films materials used for packaging skimmed milk acid coagulated cheese (Karish). Int J Biol Macromol 115:1002–1011

    CAS  PubMed  Google Scholar 

  23. Artiga-Artigas M, Acevedo-Fani A, Martín-Belloso O (2017) Improving the shelf life of low-fat cut cheese using nanoemulsion-based edible coatings containing oregano essential oil and mandarin fiber. Food Control 76:1–12

    CAS  Google Scholar 

  24. Fayed B, Abood A, El-Sayed HS, Hashem AM, Mehanna NS (2018) A synbiotic multiparticulate microcapsule for enhancing inulin intestinal release and Bifidobacterium gastro-intestinal survivability. Carbohydr Polym 193:137–143

    CAS  PubMed  Google Scholar 

  25. Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, Morelli L, Canani RB, Flint HJ, Salminen S, Calder PC, Sanders ME (2014) Expert consensus document: the International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol 11(8):506

    PubMed  Google Scholar 

  26. Khalesi S, Bellissimo N, Vandelanotte C, Williams S, Stanley D, Irwin C (2019) A review of probiotic supplementation in healthy adults: helpful or hype? Eur J Clin Nutr 73(1):24–37. https://doi.org/10.1038/s41430-018-0135-9

    Article  CAS  PubMed  Google Scholar 

  27. El-Sayed SM, El-Sayed HS (2020) Production of UF-soft cheese using probiotic bacteria and Aloe vera pulp as a good source of nutrients. Ann Agric Sci 65:13–20

    Google Scholar 

  28. El-Shafei K, Elshaghabee FMF, El-Sayed HS, Kassem JM (2018) Assessment the viability properties of Lactobacillus casei strain using labneh as a carrier. Acta Sci Pol Technol Aliment 17(3):267–276

    PubMed  Google Scholar 

  29. Fijan S (2016) Antimicrobial effect of probiotics against common pathogens. In Tech, Venkateswera

    Google Scholar 

  30. Youssef AM, Assem FM, Essam M, Elaaser M, Ibrahim O, Mahmoud M, El-Salam M (2019) Development of a novel bionanocomposite material and its use in packaging of Ras cheese. Food Chem 270:467–475

    CAS  PubMed  Google Scholar 

  31. Costa MJ, Maciel LC, Teixeira JA, Vicente AA, Cerqueira MA (2018) Use of edible films and coatings in cheese preservation: opportunities and challenges. Food Res Int 107:84–92

    CAS  PubMed  Google Scholar 

  32. Pavli F, Tassou C, Nychas GJE, Chorianopoulos N (2018) Probiotic incorporation in edible films and coatings: bioactive solution for functional foods. Int J Mol Sci 19(1):150

    PubMed Central  Google Scholar 

  33. Guimarães A, Abrunhosa L, Pastrana LM, Cerqueira MA (2018) Edible films and coatings as carriers of living microorganisms: a new strategy towards biopreservation and healthier foods. Compr Rev Food Sci Food Saf 17(3):594–614

    PubMed  Google Scholar 

  34. Quirós-Sauceda AE, Ayala-Zavala JF, Olivas GI, González-Aguilar GA (2014) Edible coatings as encapsulating matrices for bioactive compounds: a review. J Food Sci Technol 51(9):1674–1685

    PubMed  PubMed Central  Google Scholar 

  35. El-Sayed SM, El-Sayed HS, Ibrahim OA, Youssef AM (2020) Rational design of chitosan/guar gum/zinc oxide biocomposites based on Roselle calyx extract for Ras cheese coating. Carbohydr Polym 239:116234

    CAS  PubMed  Google Scholar 

  36. Youssef HF, El-Naggar ME, Fouda FK, Youssef AM (2020) Antimicrobial packaging film based on biodegradable CMC/PVA-zeolite doped with noble metal cations. Food Packag Shelf 22:100378

    Google Scholar 

  37. Kowalczyk D, Kazimierczak W, Zięba E, Mężyńska M, Basiura-Cembala M, Lisiecki S, Karaś M, Baraniak B (2018) Ascorbic acid-and sodium ascorbate-loaded oxidized potato starch films: comparative evaluation of physicochemical and antioxidant properties. Carbohydr Polym 181:317–326

    CAS  PubMed  Google Scholar 

  38. El-Sayed SM (2020) Use of spinach powder as functional ingredient in the manufacture of UF-Soft cheese. Heliyon 6(1):e03278

    PubMed  PubMed Central  Google Scholar 

  39. AOAC (2007) Association of official analytical chemists. Official methods of analysis, 18th edn. Benjamin Franklin Station, Washington

    Google Scholar 

  40. Youssef AM, Malhat FM, Hakim AA, Dekany I (2017) Synthesis and utilization of poly (methyl methacrylate) nanocomposites based on modified montmorillonite. Arab J Chem 10(5):631–642

    CAS  Google Scholar 

  41. El-Shibiny S, Abd El-Gawad MA, Assem FM, El-Sayed SM (2018) The use of nano sized eggshell powder for calcium fortification of cow’s and buffalo’s milk yogurts. Acta Sci Pol Technol Aliment 17(1):37–49

    CAS  PubMed  Google Scholar 

  42. SAS (2004) Statistical analyses systems. SAS, User guide statistics. SAS Institute Inc, Cary

    Google Scholar 

  43. Mahmoud M, Abdallah NA, El-Shafei K, Tawfik NF, El-Sayed HS (2020) Survivability of alginate-microencapsulated Lactobacillus plantarum during storage, simulated food processing and gastrointestinal conditions. Heliyon 6(3):e03541

    PubMed  PubMed Central  Google Scholar 

  44. Tang Y, Zhang X, Zhao R, Guo D, Zhang J (2018) Preparation and properties of chitosan/guar gum/nanocrystalline cellulose nanocomposite films. Carbohydr Polym 197:128–136

    CAS  PubMed  Google Scholar 

  45. Vodnar DC, Pop OL, Dulf FV, Socaciu C (2015) Antimicrobial efficiency of edible films in food industry. NotulaeBotanicaeHortiAgrobotanici Cluj-Napoca 43(2):302–312

    CAS  Google Scholar 

  46. Marín A, Plotto A, Atarés L, Chiralt A (2019) Lactic acid bacteria incorporated into edible coatings to control fungal growth and maintain postharvest quality of grapes. HortScience 54(2):337–343

    Google Scholar 

  47. Dianina IB, Jrb AGO, Pimentelc TC, Hernandesa NF, Costaa GN (2019) Edible films formulated with whey protein isolate and L. casei probiotic culture: characterization and application in tomatoes and grapes. Chem Eng Trans 75:469–474

    Google Scholar 

  48. Denkova R, Goranov B, Teneva D, Denkova Z, Kostov G (2017) Antimicrobial activity of probiotic microorganisms: mechanisms of interaction and methods of examination. In: Méndez-Vilas A (ed) Antimicrobial research: novel bioknowledge and educational programs, 1st edn. Wiley, Hoboken, pp 102–112

    Google Scholar 

  49. Chichlowski M, Croom J, McBride B, Havenstein G, Koci M (2007) Metabolic and physiological impact of probiotics or direct-fedmicrobials on poultry: a brief review of current knowledge. Int J Poult Sci 6(10):694–704

    Google Scholar 

  50. Corona-Hernandez RI, Alvarez-Parrilla E, Lizardi-Mendoza J, Islas-Rubio AR, de la Rosa LA, Wall-Medrano A (2013) Structural stability and viability of microencapsulated probiotic bacteria: a review. Compr Rev Food Sci Food Saf 12(6):614e628

    Google Scholar 

  51. Kanmani P, Lim ST (2013) Development and characterization of novel probiotic-residing pullulan/starch edible films. Food Chem 141(2):1041–1049

    CAS  PubMed  Google Scholar 

  52. El-Sisi AS, Gapr AM, Kamaly KM (2015) Use of chitosan as an edible coating in Ras cheese. Biolife 3:564–570

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Samah M. El-Sayed or Ahmed M. Youssef.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

El-Sayed, H.S., El-Sayed, S.M., Mabrouk, A.M.M. et al. Development of Eco-friendly Probiotic Edible Coatings Based on Chitosan, Alginate and Carboxymethyl Cellulose for Improving the Shelf Life of UF Soft Cheese. J Polym Environ 29, 1941–1953 (2021). https://doi.org/10.1007/s10924-020-02003-3

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10924-020-02003-3

Keywords

Navigation