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Green Synthesis of Fluorescent Carbon Dots from Annona squamosa Leaves: Optical and Structural Properties with Bactericidal, Anti-inflammatory, Anti-angiogenesis Applications

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Abstract

A hydrothermal method was employed for green synthesis of fluorescent carbon dots (GCDs) from Annona squamosa leaves. The synthesized GCDs were confirmed by microscopic and spectroscopic techniques such as: High Resolution Transmission Electron Microscopy (HR-TEM), Atomic Force Microscopy (AFM), UV–Vis spectrometry, Fluorescence spectrometry, X-Photoelectron Spectroscopy (XPS), X-ray Diffraction spectroscopy (XRD), and Fourier Transform Infrared Spectroscopy (FTIR). The produced GCDs had shown multiple properties, including massive antibacterial activity at concentration 200 μg/ml. The stabilization of human red blood cells served as a method to assess the anti-inflammatory activity. We also looked at how GCDs affected the angiogenesis process. The density of blood vessels was significantly decreased after treatment with GCDs, according to the results of the Chorio-Allantoic Membrane assay (p < 0.05). As per the study prepared GCDs from fallen leaves of Annona squamosa have multifunctional applications.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors have appreciated the support from the intramural university project (project no DYPES/DU/R&D/2021/274), D. Y. Patil Education Society, Kolhapur, India. The authors are also grateful to MJPRF given by Mahatma Jyotiba Phule Research and training Institute for financial support.

Funding

This work was supported by the D. Y. Patil Education Society, Kolhapur, India [grant number- DYPES/DU/R&D/2021/274]. The authors also acknowledge the funding support from Mylab discovery solutions pvt. ltd., Pune. 

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Authors and Affiliations

  1. Department of Medical Biotechnology and Stem Cell and Regenerative Medicine, D. Y. Patil Education Society (Deemed to Be University), Kolhapur, 416 006, Maharashtra, India

    Anuja Vibhute, Tejaswini Patil & Arpita Pandey Tiwari

  2. Optoelectronic Convergence Research Center, Department of Materials Science and Engineering, Chonnam National University, Gwangju, 61186, South Korea

    Dhanaji Malavekar

  3. Department of Electronics and Information Convergence Engineering, Kyung Hee University (Global Campus), 1732, Deogyoung Road, Giheung, Yongin, Gyeonggi, 17104, South Korea

    Shubham Patil & Seunghyun Lee

  4. Integrated Nano Electronics Laboratory, Department of Electrical Engineering, Kyung Hee University, 1732, Deogyoung Road, Giheung, Yongin, Gyeonggi, 17104, South Korea

    Seunghyun Lee

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  1. Anuja Vibhute
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  2. Tejaswini Patil
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  3. Dhanaji Malavekar
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  4. Shubham Patil
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  5. Seunghyun Lee
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  6. Arpita Pandey Tiwari
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Contributions

Dr. Arpita Tiwari conceived the presented idea. Ms. Anuja Vibhute developed the theory, carried out experiments, wrote manuscript with support from Dr. Arpita Tiwari. Ms. Tejaswini Patil helped in formal data analysis. Mr. Dhanaji Malavekar, Mr. Shubham Patil, Prof. Seunghyun Lee contributed in characterization and data analysis.

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Correspondence to Arpita Pandey Tiwari.

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Vibhute, A., Patil, T., Malavekar, D. et al. Green Synthesis of Fluorescent Carbon Dots from Annona squamosa Leaves: Optical and Structural Properties with Bactericidal, Anti-inflammatory, Anti-angiogenesis Applications. J Fluoresc 33, 1619–1629 (2023). https://doi.org/10.1007/s10895-023-03159-6

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