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Fluorescent Turn-on Aptasensor of Staphylococcus aureus Based on the FRET Between Green Carbon Quantum Dot and Gold Nanoparticle

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Abstract

This paper describes a novel strategy based on fluorescence energy transfer (FRET) with green synthesis of carbon quantum dots (CQDs) and gold nanoparticles (GNPs) for the detection of one of the most pathogenic bacteria, Staphylococcus aureus (S. aureus). Novel green CQDs with blue color emission and high quantum yield were synthesized from olive leaves via the hydrothermal method. The fluorescence of the CQDs was quenched in the presence of aptamers and GNPs. After addition of specific bacteria, the aptamer–target complex was formed and the emission of the free CQDs returned. The linear range of this aptasensor was 108 to 101 CFU/mL with a detection limit as low as 10 CFU/mL for S. aureus. The proposed aptasensing approach has been successfully used for the assay of S. aureus in human serum, milk, and orange juice. Due to its simplicity and effectiveness, this system exhibits great promise as a practical aptasensor for the detection of other bacteria and biomolecules.

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Funding

Financial support was provided by the Faculty of New Sciences and Technologies, Tehran University, Tehran (Iran). This project was funded by the Research Council of University of Tehran and authors gratefully appreciated the financial support.

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Authors and Affiliations

  1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran

    Azam Bagheri Pebdeni & Morteza Hosseini

  2. Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran, Iran

    Mohammad Reza Ganjali

  3. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Reza Ganjali

Authors
  1. Azam Bagheri Pebdeni
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  2. Morteza Hosseini
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  3. Mohammad Reza Ganjali
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Correspondence to Morteza Hosseini.

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Azam Bagheri Pebdeni·declares that there is no conflict of interest. Morteza Hosseini declares that there is no conflict of interest. Mohammad Reza Ganjali declares that there is no conflict of interest.

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Pebdeni, A.B., Hosseini, M. & Ganjali, M.R. Fluorescent Turn-on Aptasensor of Staphylococcus aureus Based on the FRET Between Green Carbon Quantum Dot and Gold Nanoparticle. Food Anal. Methods 13, 2070–2079 (2020). https://doi.org/10.1007/s12161-020-01821-4

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  • DOI: https://doi.org/10.1007/s12161-020-01821-4

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