Abstract
In this paper, a fluorescent aptamer sensor was constructed based on the carbon dots (CDs) and graphene oxide (GO). This sensor combines the excellent fluorescence performance of CDs with the high specificity of aptamer, which can detect progesterone (P4) with high sensitivity and selectivity. In the absence of P4, the CDs-aptamer system and GO form a fluorescence resonance energy transfer process (FRET), which quenches the fluorescence of the CDs. When P4 is added, the aptamer specifically binds to it, resulting the fluorescence of the CDs is recovered. At optimal conditions, the fluorescence intensity recovered by the CDs has a linear relationship with the concentration of P4 in the range of 0.1–120 nM and the detection limit is 3.3 × 10–11 M. Besides, the sensor has satisfactory detection results of P4 in milk, indicating that constructed method has enormous potential for application in food safety.
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References
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The authors thank the financial support by the National Nature Science Foundation of China (No. 21671132).
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The first author HYC performed the design, operation and data processing of the experiment, and wrote the manuscript. HL carried out the assistance of the experiment and the inspection and modification of the manuscript. YF completed the inspection and modification of the manuscript. All authors read and approved the final manuscript.
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Cui, H., Lu, H., Yang, J. et al. A Significant Fluorescent Aptamer Sensor Based on Carbon Dots and Graphene Oxide for Highly Selective Detection of Progesterone. J Fluoresc 32, 927–936 (2022). https://doi.org/10.1007/s10895-022-02896-4
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DOI: https://doi.org/10.1007/s10895-022-02896-4