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Investigation of the Interaction between 1,3-Diazaheterocyclic Compounds and the Fat Mass and Obesity-Associated Protein by Fluorescence Spectroscopy and Molecular Modeling

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Abstract

In this paper, The binding of twelve 1,3-diazaheterocyclic compounds (1a-1 l) to the fat mass and obesity-associated (FTO) protein was investigated by fluorescence, UV-vis absorption spectroscopy and molecular modeling. Results indicated that the intrinsic fluorescence of FTO is quenched by the nine compounds (1a-1i) with a static quenching procedure. No interaction was observed between FTO protein and compounds (1j-1 l). The thermodynamic parameters obtained from the fluorescence data showed that the hydrophobic force played a major role in stabilizing the complex. The results of synchronous and three-dimensional fluorescence spectra showed that the conformation of FTO was changed. In addition, the influence of molecular structure on the quenching effect has been investigated.

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Acknowledgments

We are grateful to the National Natural Science Foundation of China (No. 81330075), and 2015 Key science and technology plan project of Henan province (152102310065) for financial support.

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Authors and Affiliations

  1. College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China

    Lijiao Zhang, Ting Ren, Xianhai Tian, Zechun Wang, Wenquan Yu, Ruiyong Wang & Junbiao Chang

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  1. Lijiao Zhang
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  2. Ting Ren
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  3. Xianhai Tian
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Correspondence to Ruiyong Wang or Junbiao Chang.

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Zhang, L., Ren, T., Tian, X. et al. Investigation of the Interaction between 1,3-Diazaheterocyclic Compounds and the Fat Mass and Obesity-Associated Protein by Fluorescence Spectroscopy and Molecular Modeling. J Fluoresc 27, 369–378 (2017). https://doi.org/10.1007/s10895-016-1965-2

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  • DOI: https://doi.org/10.1007/s10895-016-1965-2

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