Abstract
In this paper, the interactions of pepsin with CYD (cytidine) or nucleoside analogs, including FNC (2′-deoxy-2′-β-fluoro-4′-azidocytidine) and CMP (cytidine monophosphate), were investigated by fluorescence, UV–visible absorption and synchronous fluorescence spectroscopy under mimic physiological conditions. The results indicated that FNC (CYD/CMP) caused the fluorescence quenching by the formation of complex. The binding constants and thermo-dynamic parameters at three different temperatures were obtained. The hydrophobic and electrostatic interactions were the predominant intermolecular forces to stabilize the complex. The F atom in FNC might weaken the binding of nucleoside analog to pepsin. Results showed that CYD was the strongest quencher and bound to pepsin with higher affinity.
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Acknowledgments
We are grateful to the National Natural Science Foundation of China (No. 81330075 and No. 21172202), and 2013 Key science and technology plan project of Henan province (132102110051) for financial support.
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Li, Z., Li, Z., Yang, L. et al. Investigation of the Binding Between Pepsin and Nucleoside Analogs by Spectroscopy and Molecular Simulation. J Fluoresc 25, 451–463 (2015). https://doi.org/10.1007/s10895-015-1532-2
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DOI: https://doi.org/10.1007/s10895-015-1532-2