Abstract
Adipose tissue-derived mesenchymal stem cells (ADSCs) have promising effects on nerve repair due to the differentiation ability to neural cells. Ghrelin has been shown to promote the neural differentiation of ADSCs. This work was designed to explore its underlying mechanism. Herein, we found high expression of LNX2 in ADSCs after neuronal differentiation. Knockdown of LNX2 might block neuronal differentiation of ADSCs, as evidenced by the decreased number of neural-like cells and dendrites per cell, and the reduced expressions of neural markers (including β-Tubulin III, Nestin, and MAP2). We also demonstrated that LNX2 silencing suppressed the nuclear translocation of β-catenin in differentiated ADSCs. Luciferase reporter assay indicated that LNX2 inhibited wnt/β-catenin pathway by reducing its transcriptional activity. In addition, results showed that LNX2 expression was increased by ghrelin, and its inhibition diminished the effects of ghrelin on neuronal differentiation. Altogether, the results suggest that LNX2 is involved in the role of ghrelin to facilitate neuronal differentiation of ADSCs.
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This research was supported by the Joint Guidance Project of Natural Science Foundation of Heilongjiang Province (grant number LH2020H074).
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Gui-Bo Liu: Conceptualization, Formal analysis, Writing-original draft, Funding acquisition.Tao Zhan: Conceptualization, Formal analysis, Writing-original draft.Yan-Ming Pan: Investigation, Formal analysis, Methodology.Da-Wei Zhang: Investigation, Formal analysis, Methodology.Hui-Zhe Zheng: Formal analysis, Methodology, Data curation.Biao Xu: Formal analysis, Methodology, Data curation.Ting-Ting Li: Software, Validation, Visualization.Chuan-Ling Dong: Software, Validation, Visualization.Yong-Xia Cheng (ORCID: 0000-0003-2652-2061): Supervision, Writing-review & editing.
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Liu, GB., Zhan, T., Pan, YM. et al. LNX2 involves in the role of ghrelin to promote the neuronal differentiation of adipose tissue-derived mesenchymal stem cells. J Bioenerg Biomembr 55, 195–205 (2023). https://doi.org/10.1007/s10863-023-09967-6
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DOI: https://doi.org/10.1007/s10863-023-09967-6