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Bone defect healing is induced by collagen sponge/polyglycolic acid

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

We have evaluated the capability of a collagen/poly glycolic acid (PGA) scaffold in regeneration of a calvarial bone defects in rabbits. 4 bone critical size defects (CSD) were created in the calvarial bone of each rabbit. The following 4 treatment modalities were tested (1) a collagen/PGA scaffold (0.52% w/w); (2) the collagen/PGA scaffold (0.52% w/w) seeded with adipose-derived mesenchymal stem cells (AD-MSCs, 1 × 106 cells per each defect); (3) AD-MSCs (1 × 106 cells) no scaffold material, and (4) blank control. The rabbits were then divided into 3 random groups (of 5) and the treatment outcomes were evaluated at 4, 8 and 12 weeks. New bone formation was histologically assessed. Experimental groups were analyzed by CT scan and real-time PCR. Histological analysis of bone defects treated with collagen/PGA alone exhibited significant fibrous connective tissue formation at the 12 weeks of treatments (P ≤ 0.05). There was no significant difference between collagen/PGA alone and collagen/PGA + AD-MSCs groups. The results were confirmed by CT scan data showing healing percentages of 34.20% for the collage/PGA group alone as compared to the control group and no difference with collagen/PGA containing AD-MSCs (1 × 106 cells). RT-PCR analysis also indicated no significant differences between collagen/PGA and collagen/PGA + AD-MSC groups, although both scaffold containing groups significantly express ALP and SIO rather than groups without scaffolds. Although there was no significant difference between the scaffolds containing cells with non-cellular scaffolds, our results indicated that the Collagen/PGA scaffold itself had a significant effect on wound healing as compared to the control group. Therefore, the collagen/PGA scaffold seems to be a promising candidate for research in bone regeneration.

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Acknowledgements

The authors are indebted to the Research Council of Mashhad University of Medical Sciences, Iran, and National Institute for Medical Research Development (NIMAD), Tehran, Iran for approval and financial support of this research.

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Authors and Affiliations

  1. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Shirin Toosi, Fatemeh Kalalinia & Javad Behravan

  2. NanoSBY Knowledge Based Corporation, Mashhad, Iran

    Shirin Toosi, Hojjat Naderi-Meshkin & Javad Behravan

  3. Stem Cells and Regenerative Medicine Research Group, Academic Center for Education Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran

    Hojjat Naderi-Meshkin, Asieh Heirani-Tabasi & Fahimeh Rezaie

  4. Innovation Center for Advanced Technology, Matrix, Inc., New York, NY, 10029, USA

    Hossein HosseinKhani

  5. Physiology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Shahrzad Havakhah

  6. Department of Radiology, Mashhad University of Medical Sciences, Mashhad, Iran

    Sirous Nekooei

  7. Department of Pathology, Mashhad University of Medical Sciences, Mashhad, Iran

    Amir Hossein Jafarian

  8. Department of Orthopedic Surgery, Orthopedic and Trauma Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Taghi Peivandi

  9. Department of Veterinary Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

    Hooman Mesgarani

  10. School of Pharmacy, University of Waterloo, Waterloo, ON, Canada

    Javad Behravan

  11. Center for Bioengineering and Biotechnology, University of Waterloo, Waterloo, ON, Canada

    Javad Behravan

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  1. Shirin Toosi
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Correspondence to Sirous Nekooei or Javad Behravan.

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Toosi, S., Naderi-Meshkin, H., Kalalinia, F. et al. Bone defect healing is induced by collagen sponge/polyglycolic acid. J Mater Sci: Mater Med 30, 33 (2019). https://doi.org/10.1007/s10856-019-6235-9

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