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In situ forming hydrogel composed of hyaluronate and polygalacturonic acid for prevention of peridural fibrosis

  • Clinical Applications of Biomaterials
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Abstract

Hyaluronic acid-based hydrogels can reduce postoperative adhesion. However, the long-term application of hyaluronic acid is limited by tissue mediated enzymatic degradation. To overcome this limitation, we developed a polygalacturonic acid and hyaluronate composite hydrogel by Schiff’s base crosslinking reaction. The polygalacturonic acid and hyaluronate composite hydrogels had short gelation time (less than 15 s) and degraded by less than 50 % in the presence of hyaluronidase for 7 days. Cell adhesion and migration assays showed polygalacturonic acid and hyaluronate composite hydrogels prevented fibroblasts from adhesion and infiltration into the hydrogels. Compared to hyaluronate hydrogels and commercial Medishield™ gels, polygalacturonic acid and hyaluronate composite hydrogel was not totally degraded in vivo after 4 weeks. In the rat laminectomy model, polygalacturonic acid and hyaluronate composite hydrogel also had better adhesion grade and smaller mean area of fibrous tissue formation over the saline control and hyaluronate hydrogel groups. Polygalacturonic acid and hyaluronate composite hydrogel is a system that can be easy to use due to its in situ cross-linkable property and potentially promising for adhesion prevention in spine surgeries.

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Acknowledgments

Support for this research was provided through National Taiwan University Hospital Hsin-Chu Branch from Grants HCH 101-19.

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Authors and Affiliations

  1. Department of Biomedical Engineering, National Yang-Ming University, No. 155, Sec. 2, Linong Street, Taipei, 112, Taiwan, ROC

    Cheng-Yi Lin, Hsiu-Hui Peng, Tse-Ying Liu & Ming-Hong Chen

  2. Department of Medicine, Far Eastern Memory Hospital, New Taipei City, Taiwan

    Mei-Hsiu Chen

  3. Department of Orthopedics, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu, Taiwan

    Jui-Sheng Sun

  4. Division of Neurosurgery, Department of Surgery, Taipei Tzu Chi Hospital, No. 289, Jinguo Rd., Xindian Dist., New Taipei City, 23142, Taiwan

    Ming-Hong Chen

  5. Department of Surgery, School of Medicine, Tzu Chi University, Hualien City, Taiwan

    Ming-Hong Chen

  6. Department of Biomedical Engineering, Ming Chuang University, Taipei, Taiwan

    Ming-Hong Chen

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  1. Cheng-Yi Lin
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Correspondence to Tse-Ying Liu or Ming-Hong Chen.

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Lin, CY., Peng, HH., Chen, MH. et al. In situ forming hydrogel composed of hyaluronate and polygalacturonic acid for prevention of peridural fibrosis. J Mater Sci: Mater Med 26, 168 (2015). https://doi.org/10.1007/s10856-015-5478-3

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  • DOI: https://doi.org/10.1007/s10856-015-5478-3

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