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Assisted oocyte activation effects on the morphokinetic pattern of derived embryos

  • Embryo Biology
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Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Objective

Assisted oocyte activation (AOA) can restore fertilization rates after IVF/ICSI cycles with fertilization failure. AOA is an experimental technique, and its downstream effects remain poorly characterized. Clarifying the relationship between AOA and embryo, morphokinetics could offer complementary insights into the quality and viability of the embryos obtained with this technique. The aim of this study is to compare the preimplantation morphokinetic development of embryos derived from ICSI-AOA (experimental group) vs. ICSI cycles (control group).

Methods

A retrospective cohort study was carried out with 141 embryos from fresh oocyte donation cycles performed between 2013 and 2017; 41 embryos were derived from 7 ICSI-AOA cycles and 100 embryos from 18 ICSI cycles. Morphokinetic development of all embryos was followed using a time-lapse system.

Results

We show that embryos from both groups develop similarly for most milestones, with the exception of the time of second polar body extrusion (tPB2) and the time to second cell division (t3).

Conclusions

We conclude that ionomycin mediated AOA does not seem to affect the morphokinetic pattern of preimplantation embryo development, despite the alterations found in tPB2 and t3, which could directly reflect the use of a Ca2+ ionophore as a transient and quick non-physiologic increase of free intracytoplasmic Ca2+.

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Acknowledgments

The authors wish to thank Désirée García and Francesc Figueras for the statistical support and to Montserrat Barragan for the helpful discussion and comments.

Funding

This study was carried out with intramural fundings from Clinica Eugin.

Author information

Authors and Affiliations

  1. Eugin, Calle Balmes 236, 08006, Barcelona, Spain

    M. Martínez, M. Durban, A. Rodríguez & R. Vassena

  2. Departamento de Biología Celular, Fisiología en Inmunología, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra, 08193, Cerdanyola del Vallès, Barcelona, Spain

    J. Santaló

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  1. M. Martínez
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Contributions

M. Martínez involved in study design, video analysis, data compilation and analysis, and manuscript preparation. M. Durban involved in study design. A. Rodriguez involved in manuscript supervision and expert knowledge. J. Santaló and R.Vassena involved in the study design, implementation and supervision, expert knowledge, and manuscript preparation.

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Correspondence to R. Vassena.

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Martínez, M., Durban, M., Santaló, J. et al. Assisted oocyte activation effects on the morphokinetic pattern of derived embryos. J Assist Reprod Genet 38, 531–537 (2021). https://doi.org/10.1007/s10815-020-02025-9

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  • DOI: https://doi.org/10.1007/s10815-020-02025-9

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