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Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles

  • Reproductive Physiology and Disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The purpose of this study was to examine the biological variability of follicular fluid (FF) high density lipoprotein (HDL) particle components measured in ipsilateral ovarian follicles.

Methods

We collected FF from two ipsilateral follicles among six women undergoing in vitro fertilization (IVF). We measured concentrations of 19 FF HDL particle components, including HDL cholesterol, free cholesterol, four cholesteryl esters, phospholipids, triglycerides, paraoxonase and arylesterase activities, apolipoproteins A-1 and A-2 (ApoA-1 and ApoA-2), and seven lipophilic micronutrients, by automated analysis and with high-performance liquid chromatography. We assessed biological variability using two-stage nested analysis of variance and compared values with those previously published for contralateral follicles.

Results

For most FF HDL analytes, there was little variability between follicles relative to the variability between women (i.e., %σ2 F:%σ2 B <0.5). Intraclass correlation coefficients were >0.80 for HDL cholesterol (0.82), phospholipids (0.89), paraoxonase (0.96), and arylesterase (0.91) activities, ApoA-1 (0.89), and ApoA-2 (0.90), and single specimen collections were required to estimate the subject-specific mean, demonstrating sufficient reliability for use as biomarkers of the follicular microenvironment in epidemiologic and clinical studies.

Conclusions

These preliminary results raise the possibility for tighter regulation of HDL in follicles within the same ovary vs. between ovaries. Thus, collection of a single FF specimen may be sufficient to estimate HDL particle components concentrations within a single ovary. However, our results should be interpreted with caution as the analysis was based on a small sample.

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Acknowledgments

The authors would like to thank Ying Wang, PhD for providing critical feedback for this manuscript.

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Authors and Affiliations

  1. Department of Environmental Health Sciences, University at Albany, State University of New York, School of Public Health Rm. #157, One University Place, Rensselaer, 12144, NY, USA

    Keewan Kim, Michael S. Bloom & Erin M. Bell

  2. Department of Epidemiology and Biostatistics, University at Albany, State University of New York, Rensselaer, NY, USA

    Michael S. Bloom & Recai M. Yucel

  3. Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California at San Francisco, San Francisco, CA, USA

    Victor Y. Fujimoto

  4. Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, State University of New York, Buffalo, NY, USA

    Richard W. Browne

Authors
  1. Keewan Kim
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  2. Michael S. Bloom
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  3. Victor Y. Fujimoto
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  4. Erin M. Bell
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  6. Richard W. Browne
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Corresponding author

Correspondence to Michael S. Bloom.

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Funding

This work was funded through grant R21 AG031957-01A2, provided by the National Institute on Aging, National Institutes of Health.

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

All procedures were performed in accordance with the ethical standards of the UCSF Committee on Human Research and with the 1964 Helsinki declaration and its later amendments. The study protocol was approved by the UCSF Committee on Human Research.

Informed consent

Informed consent was obtained prior to participation in the study.

Additional information

Capsule The variability of FF HDL analytes from ipsilateral follicles was smaller than variability that was previously reported in follicles from contralateral ovaries.

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Kim, K., Bloom, M.S., Fujimoto, V.Y. et al. Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles. J Assist Reprod Genet 33, 423–430 (2016). https://doi.org/10.1007/s10815-016-0648-x

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  • DOI: https://doi.org/10.1007/s10815-016-0648-x

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