The spectral fluorescence properties of the anionic oxacarbocyanine dye 3,3'-di-(γ-sulfopropyl)-5,5'-diphenyl-9-ethyloxacarbocyanine betaine (OCC) were studied in solutions and in complexes with human serum albumin (HSA). Interaction with HSA leads to a significant increase in the fluorescence of the dye. We studied quenching of the fluorescence of OCC in a complex with HSA by ibuprofen and warfarin. Data on quenching of fluorescence by ibuprofen indicate binding of the dye to binding site II of subdomain IIIA in the HSA molecule. Synchronous fluorescence spectra of human serum albumin in the presence of OCC showed that complexation with OCC does not lead to appreciable rearrangement of the protein molecule at the binding site.
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References
S. I. Cheger, Transport Functions of Serum Albumin [in Russian], Akademiya Rumynii, Bucharest (1975).
T. Peters, Jr., All about Albumin: Biochemistry, Genetics, and Medical Applications, Academic Press, San Diego (1996).
U. Kragh-Hansen, Pharmacol. Rev., 33, 17–53 (1981).
A. S. Tatikolov, J. Photochem. Photobiol. C: Photochem. Rev., 13, 55–90 (2012).
Er. J. Cheng, M. Vendrell, M. K. Tang, D. Zhai, and Y.-T. Chang, ACS Comb. Sci., 15, 452–457 (2013).
A. S. Tatikolov and S. M. B. Costa, Biophys. Chem., 107, 33–49 (2004).
A. S. Kashin and A. S. Tatikolov, Khim. Vysok. Énerg., 43, 536–544 (2009).
A. S. Tatikolov and I. G. Panova, Khim. Vysok. Énerg., 48, 116–122 (2014).
M. P. Samtsov, E. S. Voropay, D. G. Melnikau, L. S. Liashenka, A. A. Lugovskii, and Yu. P. Istomin, Zh. Prikl. Spektrosk., 77, No. 3, 438–444 (2010). [M. P. Samtsov, E. S. Voropay, D. G. Melnikau, L. S. Liashenka, A. A. Lugovskii, and Yu. P. Istomin, J. Appl. Spectrosc., 77, No. 3, 406–412 (2010) (English translation)].
C. A. Parker and W. T. Rees, Analyst, 85, 857–600 (1960).
G. G. Dyadyusha and A. A. Ishchenko, Zh. Prikl. Spektrosk., 30, No. 6, 1037–1042 (1979). [G. G. Dyadyusha and A. A. Ishchenko, J. Appl. Spectrosc., 30, No. 6, 746–749 (1979) (English translation)].
A. A. Ishchenko, Structure and Spectral Luminescence Properties of Polymethine Dyes [in Russian], Naukova Dumka, Kiev (1994).
G. Scatchard, Ann. N. Y. Acad. Sci., 51, 660–672 (1948).
S. D. Varfolomeev and K. G. Gurevich, Biokinetics. A Practical Course [in Russian], Fair-Press, Moscow (1999).
O. Stern and M. Volmer, Physik Zeitschr., 20, 183–188 (1919).
J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Plenum Press, New York (1983).
A. V. Hill, J. Physiol., 40, IV–VII (1910).
S. Goutelle, M. Maurin, F. Rougier, X. Barbaut, L. Bourguignon, M. Ducher, and P. Maire, Fundam. & Clinic. Pharmacol., 22, 633–648 (2008).
M. Caselli, L. Latterini, and G. Ponterini, Phys. Chem. Chem. Phys., 6, 3857–3863 (2004).
A. K. Chibisov, G. V. Zakharova, and H. Gorner, Phys. Chem. Chem. Phys., 1, 1455–1460 (1999).
D. Noukakis, M. Van der Auweraer, S. Toppet, and F. De Schryver, J. Phys. Chem., 99, 11860–11866 (1995).
A. Kober and I. Sjöholm, Mol. Pharmacol., 18, 421–426 (1980).
T. Sakai, A. Takadate, and M. Otagiri, Biol. Pharmacol. Bull., 18, 1755–1761 (1995).
G. Sudlow, D. J. Birkett, and D. N. Wade, Mol. Pharmacol., 11, 824–832 (1975).
K. Yamasaki, T. Maruyama, U. Kragh-Hansen, and M. Otagiri, Biochim. Biophys. Acta, 1295, 147–157 (1996).
J. N. Miller, Proc. Anal. Div. Chem. Soc., 16, 203–208 (1979).
D. Patra and A. K. Mishra, Trend. Analyt. Chem., 21, 787–798 (2002).
J. Hu, Y. Liu, W. Jiang, R. M. Zhao, and S. S. Qu, J. Photochem. Photobiol. B: Biol., 80, 235–242 (2005).
W. C. Abert, W. M. Gregory, and G. S. Allan, Anal. Biochem., 213, 407–413 (1993).
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 82, No. 3, pp. 429–435, May–June, 2015.
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Pronkin, P.G., Tatikolov, A.S. Spectral Fluorescence Properties of an Anionic Oxacarbocyanine Dye in Complexes with Human Serum Albumin. J Appl Spectrosc 82, 438–444 (2015). https://doi.org/10.1007/s10812-015-0126-8
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DOI: https://doi.org/10.1007/s10812-015-0126-8