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Anti-inflammatory and antinociceptive activities of a phenylpropanoid-enriched fraction of Duguetia furfuracea

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Abstract

A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf. This study aimed to obtain a phenylpropanoid-enriched fraction from the D. furfuracea (EFDf) essential oil and to investigate its anti-inflammatory and antinociceptive effects. The chemical composition of the EFDf was determined by gas chromatography–mass spectrometry (GC–MS). The in vivo anti-inflammatory activity was evaluated with a lipopolysaccharide (LPS)-induced paw oedema model. The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads. Moreover, the in vivo antinociceptive effect was assayed using the formalin test and the LPS-induced thermal hyperalgesia model. In the EFDf, 8 major compounds were identified, with α-asarone (36.4%) and 2,4,5-trimethoxystyrene (27.8%) the main constituents. A higher concentration of phenylpropanoid derivatives was found in the EFDf, 64.2% compared to the EODf (38%). The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production. The EFDf (10 and 30 mg/kg) also inhibited both phases of the formalin test and caused a significant increase in the reaction time in the LPS-induced thermal hyperalgesia model. Finally, EFDf-treated animals did not show any alteration of motor coordination. The results suggest that the enrichment of 2,4,5-trimethoxystyrene and α-asarone enhances the anti-inflammatory activity of the EFDf compared to the EODf. In contrast, the antinociception promoted by the EFDf was similar to the EODf and was mediated via activation of adenosinergic and opioidergic receptors.

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Acknowledgements

The authors are grateful to Mairon César Coimbra for his technical assistance. This study was supported by the Coordenação de Aperfeiçoamento de Pessoal do Nível Superior (CAPES) in the CAPES/PNPD program (Grant numbers 2833-2011) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Grant numbers 309054/2016-8). Aline Aparecida Saldanha thanks the Federal University of São João del-Rei for a postgraduate fellowship.

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Authors and Affiliations

  1. Laboratório de Farmacologia da Dor e Inflamação, Universidade Federal de São João del-Rei, Divinópolis, 35501-296, Brazil

    Aline Aparecida Saldanha, Letícia Vieira & Adriana Cristina Soares

  2. Laboratório de Farmacognosia/Química de Produtos Naturais, Universidade Federal de São João del-Rei, Divinópolis, Brazil

    Aline Aparecida Saldanha, Débora Soares da Silva Maia & João Máximo de Siqueira

  3. Laboratório de Biologia Molecular, Universidade Federal de São João del-Rei, Divinópolis, Brazil

    Flávio Martins de Oliveira & Débora de Oliveira Lopes

  4. Laboratório de Patologia Experimental, Universidade Federal de São João del-Rei, Divinópolis, Brazil

    Rosy Iara Maciel de Azambuja Ribeiro

  5. Laboratório de Processamento de Tecidos (LAPROTEC), Universidade Federal de São João del-Rei, Divinópolis, Brazil

    Ralph Gruppi Thomé & Hélio Batista dos Santos

  6. Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM), Universidade Federal do Mato Grosso do Sul, Campo Grande, Brazil

    Carlos Alexandre Carollo & Denise Brentan Silva

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  1. Aline Aparecida Saldanha
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Contributions

AAS and LV performed the experiments. RIMAR, RGT, and HBS analysed the data from the histological and immunohistochemistry examinations. DBS and CAC performed the GC–MS analysis. FMO and DOL performed the ELISA assay. ACS and JMS designed the study. DSSM collected the botanical material. AAS, ACS, and JMS wrote the manuscript, and all authors approved the final version.

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Correspondence to Adriana Cristina Soares.

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The authors declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the Ethics Committee in Animal Experimentation of the Federal University of São João del-Rei, Brazil (CEUA/UFSJ, protocol 016/2016).

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Saldanha, A.A., Vieira, L., Maia, D.S. et al. Anti-inflammatory and antinociceptive activities of a phenylpropanoid-enriched fraction of Duguetia furfuracea. Inflammopharmacol 29, 409–422 (2021). https://doi.org/10.1007/s10787-020-00775-7

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