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Effect of Kaempferol isolated from seeds of Eruca sativa on changes of pain sensitivity in Streptozotocin-induced diabetic neuropathy

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Abstract

Generation of excessive reactive oxygen species (ROS) and advanced glycation end products (AGEs), and cellular apoptosis are implicated in the pathogenesis of diabetic neuropathy. Present study was aimed to explore the effect of Eruca sativa and Kaempferol (KP) on hyperalgesia (thermal and mechanical); tactile allodynia, motor nerve conduction velocity (MNCV) and oxidative–nitrosative stress in streptozotocin (STZ) induced experimental diabetes. Neuropathy developed in diabetic rats was evident from a marked hyperalgesia and allodynia; reduced MNCV associated with excess formation of AGEs and ROS. Chronic treatment with E. sativa hydroalcoholic extract (EHA; 100, 200 and 400 mg/kg) and KP (5 and 10 mg/kg) for 30 days starting from the 60th day of STZ administration significantly ameliorated behavioral and biochemical changes linked to diabetic neuropathy. Present study suggested that EHA and KP corrected hyperglycemia and reversed the pain response partially in diabetic rats along via modulating oxidative and nitrosative stress along with reduction of AGEs formation in diabetic rats. Thus E. sativa might be beneficial in chronic diabetes, ameliorate the progression of diabetic neuropathy and may also find application in diabetic neuropathic pain.

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References

  • Bennett R, Rosa S, Mellon F, Kroon P (2006) Ontogenic profiling of glucosinolates, flavonoids and other secondary metabolites in Eruca sativa (salad rocket), Diplotaxis erucoides (wall rocket), Diplotaxis tenuifolia (wild rocket), and Bunias orientalis (Turkish rocket). J Agric Food Chem 54:4005–4015

    Article  PubMed  CAS  Google Scholar 

  • Beutler E, Duron O, Kelly BM (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882–888

    PubMed  CAS  Google Scholar 

  • Bhateja PK, Singh R (2014) Antidiabetic Activity of Acacia tortilis (Forsk.) Hayne ssp. raddiana Polysaccharide on Streptozotocin–Nicotinamide induced diabetic rats. BioMed Res Int 2014:9

  • Coppey LJ, Davidson EP, Dunlap JA, Lund DD, Yorek MA (2000) Slowing of motor nerve conduction velocity in streptozotocin induced diabetic rats is preceded by impaired vasodilation in arterioles that overlie the sciatic nerve. Int J Exp Diabetes Res 1:131–143

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • El-Missiry MA, El Gindy AM (2000) Amelioration of alloxan induced diabetes mellitus and oxidative stress in rats by oil of Eruca sativa seeds. Ann Nutr Metab 44:97–100

    Article  PubMed  CAS  Google Scholar 

  • Flanders A, Abdulkarim SM (1985) The composition of seed and seed oils of Taramira (Eruca sativa). JAOCS 62:1134–1135

    Article  CAS  Google Scholar 

  • Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR (1982) Analysis of nitrate, nitrite, and [15 N] nitrate in biological fluids. Anal Biochem 126:131–138

    Article  PubMed  CAS  Google Scholar 

  • Gulfraz M, Sadiq A, Tariq H, Imran M, Qureshi R, Zeenat A (2011) Phytochemical analysis and antibacterial activity of Eruca SativaSeed. Pak J Bot 43:1351–1359

    CAS  Google Scholar 

  • Ilnytska O, Lyzogubov VV, Stevens MJ, Drel VR, Mashtalir N, Pacher P, Yorek MA, Obrosova IG (2006) Poly(ADP-ribose) polymerase inhibition alleviates experimental diabetic sensory neuropathy. Diabetes 55:1686–1694

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kannan SA, Saade NE, Haddad JJ, Abdelnoor AM, Atweh SF, Jabbur SJ, Safieh-Garabedian B (1996) Endotoxin induced local inflammation and hyperalgesia in rats mince, a new model for inflammatory pain. Pain 66:373–379

    Article  Google Scholar 

  • Kaur N, Kishore L (2012) Antioxidant activity of methanolic extract of Phaseolus trilobus root powder. IJPPS 4:271–275

    CAS  Google Scholar 

  • Kaur N, Kishore L, Singh R (2016a) Antidiabetic effect of new chromane isolated from Dillenia indica L. leaves in streptozotocin induced diabetic rats. J Func Foods 22:547–555

    Article  CAS  Google Scholar 

  • Kaur N, Kishore L, Singh R (2016b) Attenuating diabetes: what really works? Curr Diabetes Rev 12:259–278

    Article  PubMed  Google Scholar 

  • Kaur N, Kishore L, Singh R (2017a) Chromane isolated from leaves of Dillenia indica improves the neuronal dysfunction in STZ-induced diabetic neuropathy. J Ethnopharmacol 206:19–30

    Article  PubMed  CAS  Google Scholar 

  • Kaur N, Kishore L, Singh R (2017b) Therapeutic effect of Linum usitatissimum L. in STZ-nicotinamide induced diabetic nephropathy via inhibition of AGE’s and oxidative stress. J Food Sci Technol 54:408–421

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kim SJ, Kawaharada C, Jin S, Hashimoto M, Ishi G, Yamauchi H (2007) Structurtal elucidation of 4-(Cysteinyl-S-yl) butyl glucosinolate from the leaves of Eruca sativa. Biosci Biotechnol Biochem 71:114–121

    Article  PubMed  CAS  Google Scholar 

  • Kim B, Choi YE, Kim HS (2014) Eruca sativa and its flavonoid components, quercetin and isorhamnetin, improve skin barrier function by activation of peroxisome proliferator-activated receptor (PPAR)-α and suppression of inflammatory cytokines. Phytother Res 28:1359–1366

    Article  PubMed  CAS  Google Scholar 

  • Kishore L, Kaur N, Singh R (2016a) Nephroprotective effect of Paeonia emodi via inhibition of advanced glycation end products and oxidative stress in streptozotocin–nicotinamide induced diabetic nephropathy. J Food Drug Anal. https://doi.org/10.1016/j.jfda.2016.08.009 (in press)

    Article  PubMed  Google Scholar 

  • Kishore L, Kaur N, Singh R (2016b) Renoprotective effect of Bacopa monnieri via inhibition of advanced glycation end products and oxidative stress in STZ-nicotinamide-induced diabetic nephropathy. Ren Fail 38:1528–1544

    Article  PubMed  CAS  Google Scholar 

  • Kishore L, Kaur N, Singh R (2016c) Distinct biomarkers for early diagnosis of diabetic nephropathy. Curr Diabetes Rev. https://doi.org/10.2174/1573399812666161207123007 (In Press)

    Article  PubMed  Google Scholar 

  • Kishore L, Kaur N, Singh R (2017) Bacosine isolated from aerial parts of Bacopa monnieri improves the neuronal dysfunction in Streptozotocin-induced diabetic neuropathy. J Func Foods 34:237–247

    Article  CAS  Google Scholar 

  • Liu J, Jia L, Kan J, Jin CH (2013) In vitro and in vivo antioxidant activity of ethanolic extract of white button mushroom (Agaricus bisporus). Food Chem Toxicol 51:310–316

    Article  PubMed  CAS  Google Scholar 

  • Mahran GH, Kadry HA, Thabet CK, Olemy MM, Al-Azizi MM, Schi PL, Wong LK (1992) GC/MS analysis of volatile oil from Eruca sativa seeds. Int J Pharmacog 30:135–137

    Article  CAS  Google Scholar 

  • Martinez-Sanchez A, Gil-Izquierdo A, Gil MI, Ferreres F (2008) A comparative study of flavonoid compounds, vitamin C, and antioxidant properties of baby leaf Brassicaceae species. J Agric Food Chem 56:2330–2340

    Article  PubMed  CAS  Google Scholar 

  • Michael HN, Shafik RE, Rasmy GE (2011) Studies on the chemical constituents of fresh leaf of Eruca sativaextract and its biological activity as anticancer agent in vitro. J Med Plant Res 5:1184–1191

    CAS  Google Scholar 

  • Morani A, Bodhankar S (2008) Neuroprotective effect of vitamin E acetate in models of mononeuropathy in rats. Neuroanatomy 7:33–37

    Google Scholar 

  • Ohkawa H, Ohishi N, Yagi K (1979) Assay of lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  PubMed  CAS  Google Scholar 

  • Perry LM (1978) Medicinal plants of East and Southeast Asia: attributed properties and uses. MIT Press, Cambridge

    Google Scholar 

  • Rani I, Akhund S, Suhail M, Abro H (2010) Antimicrobial potential of seed extract of Eruca Sativa. Pak J Bot 42:2949–2953

    Google Scholar 

  • Rice ASC, Maton S (2001) Postherpetic Neuralgia Study Group. Gabapentin in post-therapeutic neuralgia: a randomised, double blind, placebo controlled study. Pain 94:215–224

    Article  PubMed  CAS  Google Scholar 

  • Sensi M, Pricci F, Pugliese G, De Rossi MG, Petrucci AF, Cristina A, Morano S, Pozzessere G, Valle E, Andreani D, Di Mario U (1996) Role of advanced glycation end-products (AGE) in late diabetic complications. Diabetes Res Clin Pract 28:9–17

    Article  Google Scholar 

  • Sikorska M, Matławska I (2001) Kaempferol, isorhamnetin and their glycosides in the flowers of Asclepias syriaca L. Acta Pol Pharm 58:269–272

    PubMed  CAS  Google Scholar 

  • Singh R, Kaur N, Kishore L, Gupta GK (2013) Management of diabetic complications: a chemical constituents based approach. J Ethnopharmacol 150:51–70

    Article  PubMed  CAS  Google Scholar 

  • Singh R, Kishore L, Kaur N (2014) Diabetic peripheral neuropathy: current perspective and future directions. Pharmacol Res 80:21–35

    Article  PubMed  CAS  Google Scholar 

  • Tall JM, Raja SN (2004) Dietary constituents as novel therapies for pain. Clin J Pain 20:19–26

    Article  PubMed  Google Scholar 

  • Tarozzi A, Morroni F, Bolondi C, Sita G, Hrelia P, Djemil A, Cantelli-Forti G (2012) Neuroprotective effects of Erucin against 6-hydroxydopamine-induced oxidative damage in a dopaminergic-like neuroblastoma cell line. Int J Mol Sci 13:10899–10910

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • The Plant List (2016) A working list of all plant species. Webpage at: https://www.theplantlist.org

  • Uphof JCT (1968) Dictionary of Economic Plants, 2nd edn. Verlag Von J. Cramer Publ, New York, p 591

    Google Scholar 

  • Wang HD, Pagano PJ, Du Y, Cayatte AJ, Quinn MT, Brecher P, Cohen RA (1998) Superoxide anion from the adventitia of the rat thoracic aorta inactivates nitric oxide. Circ Res 82:810–818

    Article  PubMed  CAS  Google Scholar 

  • Weckerle B, Michel K, Balazs B, Schreier P, Toth G (2001) Quercetin 3,30,40-tri-O-b-d-glucopyranosides from leaves of Eruca sativa (Mill.). Phytochemistry 57:547–551

    Article  PubMed  CAS  Google Scholar 

  • Yaniv Z, Schafferman D, Amar Z (1998) Tradition, uses, and biodiversity of rocket (Eruca sativa) in Israel. Econ Bot 52:394–400

    Article  Google Scholar 

Download references

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  1. M.M. College of Pharmacy, M.M. University, Mullana, Ambala, Haryana, 133207, India

    Lalit Kishore, Navpreet Kaur & Randhir Singh

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  1. Lalit Kishore
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  2. Navpreet Kaur
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  3. Randhir Singh
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Correspondence to Randhir Singh.

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Kishore, L., Kaur, N. & Singh, R. Effect of Kaempferol isolated from seeds of Eruca sativa on changes of pain sensitivity in Streptozotocin-induced diabetic neuropathy. Inflammopharmacol 26, 993–1003 (2018). https://doi.org/10.1007/s10787-017-0416-2

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  • DOI: https://doi.org/10.1007/s10787-017-0416-2

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